Catechin Isolated from Garcinia celebica Leaves Inhibit Plasmodium falciparum Growth through the Induction of Oxidative Stress.
Pharmacogn Mag
; 13(Suppl 2): S301-S305, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28808396
ABSTRACT
BACKGROUND:
Resistance of antimalarial drugs to Plasmodium falciparum has become a major concern in malaria eradication. Although it is also affected by several socioeconomic factors, a new antiplasmodial agent is needed for a global malaria control program.OBJECTIVE:
In this study, we attempted to uncover the antiplasmodial properties of Garcinia celebica, an Indonesian medicinal plant, along with the responsible compound and its possible mechanism. MATERIALS ANDMETHODS:
The G. celebica leaves were ethanol extracted and fractionated based on their polarity using n-hexane, ethyl acetate, and water. The antiplasmodial activity was tested in vitro against chloroquine-resistant P. falciparum at 100 µg/ml for 72 h. The active compound of the most active ethyl acetate fraction was subsequently isolated using column chromatography and identified by nuclear magnetic resonance.RESULTS:
The IC50 of (+)-catechin, the characterized compound, against P. falciparum was 198 µM in 24 h and experiment. The isolated catechin inhibited P. falciparum growth in both trophozoite and schizont stages. An additional experiment also suggests that the antiplasmodial property of catechin occurs through the induction of the oxidative stress to P. falciparum.CONCLUSION:
This result shows that the potential of catechin and its antimalarial properties should be explored further.SUMMARY:
Garcinia celebica leaf extract and fractions inhibit Plasmodium falciparum growthCatechin, the active compound of Garcinia celebica leaf extract, inhibits Plasmodium falciparum growth in a time- and dose-dependent manner Abbreviations used RBC Red Blood Cells; IC50 Inhibition Concentrattino 50; MeOH Methanol; RPMI Roswell Park Memorial Institute; EI Electron Ionization.
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Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Pharmacogn Mag
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Indonésia