Involvement of pentraxin-3 in anti-neutrophil cytoplasmic antibody production induced by aluminum salt adjuvant.
Clin Exp Rheumatol
; 35(5): 735-738, 2017.
Article
em En
| MEDLINE
| ID: mdl-28850023
ABSTRACT
OBJECTIVES:
Pentraxin 3 (PTX3) is a multifunctional soluble factor. PTX3 can be involved in the regulation of vasculitis and is expressed in the cytoplasm of neutrophils. As anti-neutrophil cytoplasmic antibody (ANCA) is recognised as a cause of vasculitis, we aimed to discover the role of PTX3 in ANCA production in vivo.METHODS:
To this end, we used aluminum salt (alum), which induces neutrophil extracellular traps, as an adjuvant for producing anti-myeloperoxidase-ANCA (MPO-ANCA). Specifically, we intraperitoneally injected alum and recombinant MPO (rMPO) into MPO-deficient mice and then measured the concentration of anti-MPO IgG in their blood. To show the involvement of extracellular PTX3 in this model, we assessed PTX3 protein content and host double-stranded DNA levels in the mice's peritoneal fluid after alum injection. In addition, we simultaneously administered recombinant PTX3, rMPO and alum to MPO-deficient mice to assess the function of PTX3 in producing anti-MPO IgG in vivo.RESULTS:
Anti-MPO IgG was produced by the alum + rMPO immunisation model in MPO-deficient but not wildtype mice. Injection of alum induced extracellular PTX3 as well as double-stranded DNA and dead cells in MPO-deficient mice. Simultaneous injection of recombinant PTX3 with rMPO and alum attenuated the production of anti-MPO IgG in MPO-deficient mice.CONCLUSIONS:
Our current findings provide evidence that PTX3 attenuates the production of murine MPO-ANCA.
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Base de dados:
MEDLINE
Assunto principal:
Proteína C-Reativa
/
Imunoglobulina G
/
Adjuvantes Imunológicos
/
Peroxidase
/
Anticorpos Anticitoplasma de Neutrófilos
/
Compostos de Alúmen
/
Erros Inatos do Metabolismo
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Clin Exp Rheumatol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Japão