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Correlation of Somatostatin Receptor-2 Expression with Gallium-68-DOTA-TATE Uptake in Neuroblastoma Xenograft Models.
Zhang, Libo; Vines, Douglass C; Scollard, Deborah A; McKee, Trevor; Komal, Teesha; Ganguly, Milan; Do, Trevor; Wu, Bing; Alexander, Natasha; Vali, Reza; Shammas, Amer; Besanger, Travis; Baruchel, Sylvain.
Afiliação
  • Zhang L; The Hospital for Sick Children, Toronto, ON, Canada.
  • Vines DC; The STTARR Innovation Centre, University Health Network, Toronto, ON, Canada.
  • Scollard DA; The STTARR Innovation Centre, University Health Network, Toronto, ON, Canada.
  • McKee T; The STTARR Innovation Centre, University Health Network, Toronto, ON, Canada.
  • Komal T; The STTARR Innovation Centre, University Health Network, Toronto, ON, Canada.
  • Ganguly M; The STTARR Innovation Centre, University Health Network, Toronto, ON, Canada.
  • Do T; The STTARR Innovation Centre, University Health Network, Toronto, ON, Canada.
  • Wu B; The Hospital for Sick Children, Toronto, ON, Canada.
  • Alexander N; The Hospital for Sick Children, Toronto, ON, Canada.
  • Vali R; The Hospital for Sick Children, Toronto, ON, Canada.
  • Shammas A; The Hospital for Sick Children, Toronto, ON, Canada.
  • Besanger T; The Center for Probe Development and Commercialization, Hamilton, ON, Canada.
  • Baruchel S; The Hospital for Sick Children, Toronto, ON, Canada.
Contrast Media Mol Imaging ; 2017: 9481276, 2017.
Article em En | MEDLINE | ID: mdl-29097943
ABSTRACT
Peptide-receptor imaging and therapy with radiolabeled somatostatin analogs such as 68Ga-DOTA-TATE and 177Lu-DOTA-TATE have become an effective treatment option for SSTR-positive neuroendocrine tumors. The purpose of this study was to evaluate the correlation of somatostatin receptor-2 (SSTR2) expression with 68Ga-DOTA-TATE uptake and 177Lu-DOTA-TATE therapy in neuroblastoma (NB) xenograft models. We demonstrated variable SSTR2 expression profiles in eight NB cell lines. From micro-PET imaging and autoradiography, a higher uptake of 68Ga-DOTA-TATE was observed in SSTR2 high-expressing NB xenografts (CHLA-15) compared to SSTR2 low-expressing NB xenografts (SK-N-BE(2)). Combined autoradiography-immunohistochemistry revealed histological colocalization of SSTR2 and 68Ga-DOTA-TATE uptake in CHLA-15 tumors. With a low dose of 177Lu-DOTA-TATE (20 MBq/animal), tumor growth inhibition was achieved in the CHLA-15 high SSTR2 expressing xenograft model. Although, in vitro, NB cells showed variable expression levels of norepinephrine transporter (NET), a molecular target for 131I-MIBG therapy, low 123I-MIBG uptake was observed in all selected NB xenografts. In conclusion, SSTR2 expression levels are associated with 68Ga-DOTA-TATE uptake and antitumor efficacy of 177Lu-DOTA-TATE. 68Ga-DOTA-TATE PET is superior to 123I-MIBG SPECT imaging in detecting NB tumors in our model. Radiolabeled DOTA-TATE can be used as an agent for NB tumor imaging to potentially discriminate tumors eligible for 177Lu-DOTA-TATE therapy.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Receptores de Somatostatina / Radioisótopos de Gálio / Neuroblastoma Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Contrast Media Mol Imaging Assunto da revista: DIAGNOSTICO POR IMAGEM Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Canadá
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Receptores de Somatostatina / Radioisótopos de Gálio / Neuroblastoma Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Contrast Media Mol Imaging Assunto da revista: DIAGNOSTICO POR IMAGEM Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Canadá