A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils.
Colloids Surf B Biointerfaces
; 165: 28-36, 2018 May 01.
Article
em En
| MEDLINE
| ID: mdl-29448217
ABSTRACT
Herein, we report a novel concept of low-cost flexible platform for fluorescence-based biosensor. The surface of polyethylene naphthalate (PEN) foil was exposed to KrF excimer laser through a photolitographic contact mask. Laser initiated surface modification resulted in micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. High affinity binding protein (albumin-binding domain (ABD) of protein G, Streptococcus G148) recognizing human serum albumin (HSA), genetically fused with streptavidin (SA-ABDwt), was immobilized on the micro-patterned surface through biotin-streptavidin coupling. Fluorescently labelled HSA analyte was detected in several blocking environments, in 1% bovine serum albumin (BSA) and 6% fetal serum albumin (FBS), respectively. We conclude that the presented novel concept enabled us to micropattern functional biosensing layers on the surface of PEN foil in a fast and easy way. It brings all necessary aspects for continuous roll-to-roll fabrication of low-cost optical bioanalytical devices.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polietilenos
/
Fenômenos Ópticos
/
Microtecnologia
/
Naftalenos
Tipo de estudo:
Clinical_trials
Limite:
Humans
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Assunto da revista:
QUIMICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
República Tcheca