Decoy receptor 3 alleviates hepatic fibrosis through suppressing inflammation activated by NF-κB signaling pathway.
Adv Clin Exp Med
; 27(4): 441-447, 2018 Apr.
Article
em En
| MEDLINE
| ID: mdl-29558040
ABSTRACT
BACKGROUND:
Hepatic fibrosis is a reversible pathological process. Inflammatory responses are the prevailing reactions during hepatic fibrosis. Decoy receptor 3 (DcR3) has been reported to have an anti-inflammatory effect.OBJECTIVES:
The aim of the study was to investigate the preventive effects of DcR3 on hepatic fibrosis. MATERIAL ANDMETHODS:
Hepatic fibrosis was induced in rats by administering intraperitoneally (ip.) 1% dimethylnitrosamine (DMN). DcR3 plasmid was delivered into rats by intravenous injection. After 4 weeks, the expression of DcR3, TNF-like molecule 1A (TL1A) and α-SMA of the liver tissue were checked. The levels of inflammatory cytokines such as TNF-α, IL-6 and IL-1ß were detected using western blotting and quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Masson's trichrome staining for histopathological changes of the liver tissue was observed. Finally, the activity of NF-κB in the liver was examined by enzyme-linked immunosorbent assay (ELISA).RESULTS:
A higher expression of DcR3 was observed in rats treated with DcR3 (p < 0.05). Histological results showed that DcR3 significantly attenuated pathology in hepatic fibrosis rats. Consistently, mRNA and protein levels of α-SMA, TL1A, TNF-α, IL-6, and IL-1ß were repressed in the liver tissue after treatment with DcR3 (p < 0.05). Moreover, DcR3 also inhibited the activation of NF-κB in the liver tissue (p < 0.05).CONCLUSIONS:
This study demonstrated that DcR3 attenuated liver injury and inflammatory responses in rats with hepatic fibrosis. We suggest DcR3 may be a prophylactic and promising therapeutic agent in the treatment of hepatic fibrosis.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
NF-kappa B
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Membro 6b de Receptores do Fator de Necrose Tumoral
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Inflamação
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Cirrose Hepática
Limite:
Animals
Idioma:
En
Revista:
Adv Clin Exp Med
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China