Comparison of two commercial quality control sera for adrenocorticotropin (ACTH) used in Elecsys® immunoassay system.
J Clin Lab Anal
; 33(1): e22618, 2019 Jan.
Article
em En
| MEDLINE
| ID: mdl-30006935
ABSTRACT
OBJECTIVES:
The purpose of our study was to investigate whether the storage time and temperature of internal quality control (IQC) material influence the result of ACTH in IQC measurements. DESIGN ANDMETHODS:
Five levels of IQC materials from two manufacturers were tested through the precision of ACTH, the three freeze/thaw cycles, and the storage time and temperature to evaluate the stability of IQC material. All commercial control materials were simultaneously tested three times a day for five consecutive days.RESULTS:
Total precision of three levels of Bio-Rad IQC sera was 13.93%, 16.45%, and 15.98%, respectively, but repeatability was <2%. The concentration of ACTH decreased by 30%-50% after 3 freeze/thaw cycles. At room temperature, the concentration of ACTH from 3 levels decreased by 16.60%, 17.98%, and 17.20%, respectively, after 0.5 hours, and 70.54%, 74.36%, and 72.03%, respectively, after 4 hours. However, after 2 hours of storage at 4°C, the decline in the measured ACTH IQC was 8.04%, 11.84%, and 10.11%, respectively. Total precision of Roche IQC was 1.17% and 1.08%, respectively. After 3 freeze/thaw cycles, the concentration of ACTH decreased <5%. After 4 hours, the change of ACTH still steadied within 5% both at the room temperature and at 4°C.CONCLUSION:
Roche is a better choice for ACTH of IQC material in Elecsys® immunoassay system in our study. If Bio-Rad control materials be used in Elecsys® immunoassay system for ACTH IQC testing material, it should be stored at 4°C and testing should be completed within 1 hours.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Hormônio Adrenocorticotrópico
Limite:
Humans
Idioma:
En
Revista:
J Clin Lab Anal
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China