Interleukin-7 improves in vitro maturation of ovine cumulus-oocyte complexes in a dose dependent manner.
Cytokine
; 113: 296-304, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30049453
ABSTRACT
Interleukin-7 (IL-7) mediated signals are linked to development, proliferation, survival and differentiation of cells. Recent evidences indicate its role in oocyte maturation process as well. Nevertheless, the underlying mechanisms of IL-7 involvement in oocyte maturation are not well characterized. In addition, currently no information is available on the effect of exogenous IL-7 on oocyte maturation in ovine or any other species. In this study, the effect of IL-7 supplementation during in vitro maturation (IVM) on the maturation rate, production of reactive oxygen species (ROS) and gene expression of ovine cumulus-oocyte complexes (COC) was assessed. IL-7 (0.5, 1, 2, 5 and 10â¯ng/ml) was supplemented in IVM medium at the beginning (0â¯h) and maturation rate of COC was assessed at the completion of IVM (24â¯h). The maturation rate (%) was found significantly (Pâ¯=â¯0.000) greater with the 1â¯ng/ml of IL-7 supplementation (69.5) than control (60.0). In contrast, the maturation rate was reduced significantly (Pâ¯=â¯0.000) with the 2 (47.1), 5 (39.2) and 10â¯ng/ml (39.1) of IL-7 as compared to the control. The level of intracellular ROS in the matured COC was found considerably higher with the 5â¯ng/ml of IL-7 followed by 1â¯ng/ml of IL-7 and control. It was evident that in the presence of superoxide dismutase-inhibitor, 1â¯ng/ml of IL-7 did not stimulate oocyte maturation. In contrast, oocyte maturation was improved with 5â¯ng/ml of IL-7 supplementation in the presence of NADPH-oxidase-inhibitor. IL-7 supplementation influenced gene expression in COC in a dose and time dependant manner. The expression of genes related to ROS production and apoptosis were upregulated and the genes associated with antioxidant mechanisms were downregulated noticeably with the supplementation of 5â¯ng/ml of IL-7. In conclusion, IL-7 at low concentration was beneficial for oocyte maturation, which was likely mediated through the favourable level of intracellular ROS and antioxidant mechanisms. In contrast, the detrimental effects of greater IL-7 concentrations on oocyte maturation were possibly arbitrated through the ROS-mediated oxidative stress, compromised antioxidant mechanism and stimulated apoptotic signalling.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Transdução de Sinais
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Interleucina-7
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Espécies Reativas de Oxigênio
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Apoptose
Limite:
Animals
Idioma:
En
Revista:
Cytokine
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Índia