A new algorithm for high-resolution reconstruction of single particles by electron microscopy.
J Struct Biol
; 204(2): 329-337, 2018 11.
Article
em En
| MEDLINE
| ID: mdl-30145327
ABSTRACT
The Map Challenge organized by the Electron Microscopy Data Bank has prompted the development of an Xmipp high resolution reconstruction protocol (which we will refer to as highres) that is integrated in the software platform Scipion. In this work we describe the details of the image angular alignment and map reconstruction steps in our new method. This algorithm is similar to the standard projection matching approach with some important modifications, especially in the area of detecting significant features in the reconstructed volume. We show that the new method is able to produce higher resolution maps than the current de facto standard as measured by the Fourier Shell Correlation, the Monogenic Local Resolution and EMRinger.
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Base de dados:
MEDLINE
Assunto principal:
Microscopia Eletrônica
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Struct Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2018
Tipo de documento:
Article