Mutation-induced alterations of intra-filament subunit organization in vimentin filaments revealed by SAXS.
Soft Matter
; 15(9): 1999-2008, 2019 Feb 27.
Article
em En
| MEDLINE
| ID: mdl-30719518
ABSTRACT
Vimentin intermediate filaments constitute a distinct filament system in mesenchymal cells that is instrumental for cellular mechanics and migration. In vitro, the rod-like monomers assemble in a multi-step, salt-dependent manner into micrometer long biopolymers. To disclose the underlying mechanisms further, we employed small angle X-ray scattering on two recombinant vimentin variants, whose assembly departs at strategic points from the normal assembly route (i) vimentin with a tyrosine to leucine change at position 117; (ii) vimentin missing the non-α-helical carboxyl-terminal domain. Y117L vimentin assembles into unit-length filaments (ULFs) only, whereas ΔT vimentin assembles into filaments containing a higher number of tetramers per cross section than normal vimentin filaments. We show that the shape and inner structure of these mutant filaments is significantly altered. ULFs assembled from Y117L vimentin contain more, less tightly bundled vimentin tetramers, and ΔT vimentin filaments preserve the number density despite the higher number of tetramers per filament cross-section.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vimentina
/
Filamentos Intermediários
/
Subunidades Proteicas
/
Mutação
Limite:
Humans
Idioma:
En
Revista:
Soft Matter
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Alemanha