Kinetic Analysis of Phagosomal ROS Generation.
Methods Mol Biol
; 1982: 301-312, 2019.
Article
em En
| MEDLINE
| ID: mdl-31172480
ABSTRACT
Phagosomal ROS generation is critical for our immune defense against microbial infections. Quantitative assessment of phagosomal ROS production is required to understand the complex relationship between the phagocyte and the microbe, in particular for pathogens that resist phagosomal destruction. ROS detection is difficult due to the transient nature of the reactive species and their multiple interactions with the environment. Direct labeling of phagocytic prey with a ROS-sensitive dye allows to target the dye into the phagosome and to follow the kinetics of phagosomal ROS production on a single phagosome base. Here we describe the basic labeling procedure, the quality assessment, and the imaging technique to achieve this kinetic analysis.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fagossomos
/
Espécies Reativas de Oxigênio
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
França