The planarian Vinculin is required for the regeneration of GABAergic neurons in Dugesia japonica.
Exp Cell Res
; 383(1): 111540, 2019 10 01.
Article
em En
| MEDLINE
| ID: mdl-31369753
ABSTRACT
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, playing an important role in linkage of integrin adhesion molecules to the actin cytoskeleton. The planarian nervous system is a fascinating system for studying the organogenesis during regeneration. In this paper, a homolog gene of Vinculin, DjVinculin, was identified and characterized in Dugesia japonica. The DjVinculin sequence analysis revealed that it contains an opening reading frame encoding a putative protein of 975 amino acids with functionally domains that are highly conserved, including eight anti-parallel α-helical bundles organized into five distinct domains. Whole mount in situ hybridization showed that DjVinculin was predominantly expressed in the brain of intact and regenerating planarians. RNA interference of DjVinculin caused distinct defects in brain morphogenesis and influences the regeneration of planarian GABAergic neurons. The expression level of DjGAD protein was decreased in the DjVinculin-knockdown planarians. These findings suggest that DjVinculin is required for GABAergic neurons regeneration.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Planárias
/
Regeneração
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Proteínas de Helminto
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Vinculina
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Regulação da Expressão Gênica no Desenvolvimento
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Neurônios GABAérgicos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China