Differentiation of Neural Crest Stem Cells in Response to Matrix Stiffness and TGF-ß1 in Vascular Regeneration.
Stem Cells Dev
; 29(4): 249-256, 2020 02 15.
Article
em En
| MEDLINE
| ID: mdl-31701817
ABSTRACT
The neural crest stem cells derived from human induced pluripotent stem cells (iPSC-NCSCs) are a valuable autologous cell source for tissue engineering and regenerative medicine. In this study, we investigated how iPSC-NCSCs could be regulated to regenerate arteries by microenvironmental factors, including the physical factor of matrix stiffness, and the chemical factor of transforming growth factor beta-1 (TGF-ß1). We found that, compared to soft substrate, stiff substrate drove iPSC-NCSCs differentiation into smooth muscle cells, which was further enhanced by TGF-ß1. To investigate the regulatory role of TGF-ß1 in vivo, we fabricated vascular grafts composed of electrospun nanofibrous scaffolds, collagen gel, iPSC-NCSCs, and TGF-ß1, and implanted them into athymic rats. The results showed that TGF-ß1 significantly promoted extracellular matrix synthesis and increased mechanical strength of vascular grafts. This study presents a proof of concept that iPSC-NCSCs can be used as a promising autologous cell source for vascular regeneration when combined with physical and chemical engineering.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Prótese Vascular
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Artérias Carótidas
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Fator de Crescimento Transformador beta1
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Alicerces Teciduais
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Células-Tronco Pluripotentes Induzidas
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Células-Tronco Neurais
Limite:
Animals
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Humans
Idioma:
En
Revista:
Stem Cells Dev
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China