[Identification of cryptic structural chromosomal aberrations in parents through detection of copy number variations in miscarriage tissues].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 36(11): 1123-1126, 2019 Nov 10.
Article
em Zh
| MEDLINE
| ID: mdl-31703141
ABSTRACT
OBJECTIVE:
To explore the genetic cause for abnormal pregnancies through detecting chromosomal copy number variations (CNVs) in abortic tissues by next generation sequencing (NGS).METHODS:
NGS technique was used to detect CNVs in abortion tissues. Parental chromosomal karyotypes were predicted based on the results. The aberrant chromosomal segments of the parents were accurately mapped by G-banding karyotyping analysis and fluorescence in situ hybridization (FISH).RESULTS:
In addition to numerical chromosomal aberrations, 12 microdeletion/microduplications were detected by NGS. For 8 families where both parents accepted chromosomal karyotyping, 4 carriers of chromosomal abnormalities were identified. One marker chromosome was missed by karyotyping analysis, and a mother was confirmed to carry a cryptic balanced translocation by FISH.CONCLUSION:
NGS can facilitate detection of cryptic chromosomal translocations in couples with repeated pregnancy failure and is of great value for detecting abnormal CNVs for its high sensitivity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Aborto Espontâneo
/
Aberrações Cromossômicas
/
Variações do Número de Cópias de DNA
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
Zh
Revista:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Assunto da revista:
GENETICA MEDICA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China