Inducing inflammatory response in RAW264.7 and NK-92 cells by an arabinogalactan isolated from Ferula gummosa via NF-κB and MAPK signaling pathways.
Carbohydr Polym
; 241: 116358, 2020 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-32507213
ABSTRACT
The polysaccharide isolated from F. gummosa (FGP) was found homogenous with a weight average molecular weight (Mw) of 50.0â¯×â¯103 g/mol and radius of gyration (Rg) of 105.3â¯nm. The FGP was an arabinogalactan with a backbone formed of â6)-ß-Galp-1â residues having random branching points at C-3 extended with either ß-Galp-(1â3)-ß-Galp-(1â or α-Araf-(1â side chain residues. FGP exhibited proliferative effect on RAW264.7 cells and induced macrophages to exert proinflammatory response releasing NO and up-regulating the transcription of cytokines including TNF-α, IL-1ß, IL-6 and IL-12. The FGP induced NK-92 cells to up-regulate the expressions of TNF-α, IFN-γ, granzyme-B, perforin, NKG2D and FasL. The presence of p-NF- κB, p-ERK, p-JNK and p-p38 in RAW264.7 and NK-92 cells indicated their activation through NF-κB and MAPKs signaling pathways. These findings suggested that polysaccharides from F. gummosa are potent in boosting immune system and thus may be considered for further studies of biomedical applications.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Ferula
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Galactanos
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Fatores Imunológicos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2020
Tipo de documento:
Article