Oxiapoptophagy: A type of cell death induced by some oxysterols.
Br J Pharmacol
; 178(16): 3115-3123, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-32579703
ABSTRACT
Oxysterols are oxidized forms of cholesterol generated from cholesterol by auto-oxidation, enzymatic processes, or both. Some of them (7-ketocholesterol, 7ß-hydroxycholesterol and 24(S)-hydroxycholesterol), when used at cytotoxic concentrations on different cell types from different species (mesenchymal bone marrow cells, monocytic cells and nerve cells), induce a type of cell death associated with OXIdative stress and several characteristics of APOPTOsis and autoPHAGY, defined as oxiapoptophagy. Oxidative stress is associated with overproduction of ROS, increased antioxidant enzyme activities, lipid peroxidation and protein carbonylation. Apoptosis is associated with activation of the mitochondrial pathway, opening of the mitochondrial permeability pore, loss of mitochondrial membrane potential, caspase-3 activation, PARP degradation, nuclear condensation and/or fragmentation. Autophagy is characterized by autophagic vacuoles revealed by monodansylcadaverine staining and transmission electron microscopy, plus increased ratio of LC-3II/LC-3I. In addition, morphological, topographical and functional changes of the peroxisome are observed. LINKED ARTICLES This article is part of a themed issue on Oxysterols, Lifelong Health and Therapeutics. To view the other articles in this section visit http//onlinelibrary.wiley.com/doi/10.1111/bph.v178.16/issuetoc.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxisteróis
Idioma:
En
Revista:
Br J Pharmacol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
França