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Evaluation of the In Vitro Damage Caused by Lipid Factors on Stem Cells from a Female Rat Model of Type 2 Diabetes/Obesity and Stress Urinary Incontinence.
Kovanecz, Istvan; Gelfand, Robert; Sharifzad, Sheila; Ohanian, Alec; DeCastro, William Brent; Cooper, Carley; Lin, Guiting; Lue, Tom; Gonzalez-Cadavid, Nestor F.
Afiliação
  • Kovanecz I; Division of Urology, Department of Surgery, Harbor-UCLA Medical Center and The Lundquist Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
  • Gelfand R; Department of Urology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1768, USA.
  • Sharifzad S; Division of Urology, Department of Surgery, Harbor-UCLA Medical Center and The Lundquist Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
  • Ohanian A; Department of Medicine, Charles Drew University of Medicine and Science, Los Angeles, CA 90059, USA.
  • DeCastro WB; Division of Urology, Department of Surgery, Harbor-UCLA Medical Center and The Lundquist Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
  • Cooper C; Division of Urology, Department of Surgery, Harbor-UCLA Medical Center and The Lundquist Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
  • Lin G; Division of Urology, Department of Surgery, Harbor-UCLA Medical Center and The Lundquist Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
  • Lue T; Department of Medicine, Charles Drew University of Medicine and Science, Los Angeles, CA 90059, USA.
  • Gonzalez-Cadavid NF; Division of Urology, Department of Surgery, Harbor-UCLA Medical Center and The Lundquist Institute at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
Int J Mol Sci ; 21(14)2020 Jul 17.
Article em En | MEDLINE | ID: mdl-32708907
ABSTRACT
Human stem cell therapy for type 2 diabetes/obesity (T2D/O) complications is performedwith stem cell autografts, exposed to the noxious T2D/O milieu, often with suboptimal results.We showed in the Obese Zucker (OZ) rat model of T2D/O that when their muscle-derived stemcells (MDSC) were from long-term T2D/O male rats, their repair ecacy for erectile dysfunctionwas impaired and were imprinted with abnormal gene- and miR-global transcriptional signatures(GTS). The damage was reproduced in vitro by short-term exposure of normal MDSC to dyslipidemicserum, causing altered miR-GTS, fat infiltration, apoptosis, impaired scratch healing, and myostatinoverexpression. Similar in vitro alterations occurred with their normal counterparts (ZF4-SC) fromthe T2D/O rat model for female stress urinary incontinence, and with ZL4-SC from non-T2D/O leanfemale rats. In the current work we studied the in vitro eects of cholesterol and Na palmitate aslipid factors on ZF4-SC and ZL4-SC. A damage partially resembling the one caused by the femaledyslipidemic serum was found, but diering between both lipid factors, so that each one appears tocontribute specifically to the stem cell damaging eects of dyslipidemic serum in vitro and T2D/Oin vivo, irrespective of gender. These results also confirm the miR-GTS biomarker value forMDSC damage.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Incontinência Urinária por Estresse / Colesterol / Ácido Palmítico / Diabetes Mellitus Tipo 2 / Obesidade Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Incontinência Urinária por Estresse / Colesterol / Ácido Palmítico / Diabetes Mellitus Tipo 2 / Obesidade Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos