Molecular and serological characterization of occult hepatitis B among blood donors in Maputo, Mozambique.
Mem Inst Oswaldo Cruz
; 115: e200006, 2020.
Article
em En
| MEDLINE
| ID: mdl-32997000
ABSTRACT
BACKGROUND:
Occult hepatitis B virus (HBV) - characterized by the absence of detectable HBsAg in the presence of HBV DNA - represents a potential threat for blood safety.OBJECTIVES:
This study was conducted with the aim to investigate the serological and molecular characterization of occult HBV infection (OBI) among blood donors in Mozambique.METHODS:
1,502 blood donors were tested for HBsAg. All HBsAg-negative individuals were tested for HBV DNA. Antibodies against HBV core, surface and HBe antigen (anti-HBc, anti-HBs, HBeAg) were measured in HBV DNA positive individuals.FINDINGS:
1435 serum samples were HBsAg negative and 16 positive for HBV DNA, 14 confirmed to have OBI, corresponding to a frequency of 0.98%. Of the 14 OBI infections identified, 13/14 (92.8%) were positive for anti-HBc, 4/14 (28.5%) for anti-HBs, and no samples were reactive for HBeAg. Of the 14 OBI cases, nine samples (64.2%) were sequenced for the S/P region. Eight samples (88.9%) belonged to genotype A1 and one (11.1%) to genotype E. One escape mutation (T123A) associated with OBI and various amino acid substitutions for genotype A1 and E were observed. MAINCONCLUSIONS:
Our results show the importance of using nucleic acid amplification test to detect occult hepatitis B infection in blood donors in Mozambique.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doadores de Sangue
/
Vírus da Hepatite B
/
Técnicas de Amplificação de Ácido Nucleico
/
Hepatite B
/
Antígenos de Superfície da Hepatite B
Tipo de estudo:
Observational_studies
/
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
País/Região como assunto:
Africa
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Moçambique