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A 3D In Vitro Model of the Human Airway Epithelium Exposed to Tritiated Water: Dosimetric Estimate and Cytotoxic Effects.
Baiocco, Giorgio; George, Isabelle; Garcia-Argote, Sébastien; Guardamagna, Isabella; Lonati, Leonardo; Lamartinière, Yordenca; Orsière, Thierry; Rousseau, Bernard; Ottolenghi, Andrea; Jha, Awadhesh; Lebaron-Jacobs, Laurence; Grisolia, Christian; Malard, Véronique.
Afiliação
  • Baiocco G; Laboratory of Radiation Biophysics and Radiobiology, Department of Physics, University of Pavia, Pavia, Italy.
  • George I; Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SCBM, F-91191, Gif-sur-Yvette, France.
  • Garcia-Argote S; Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SCBM, F-91191, Gif-sur-Yvette, France.
  • Guardamagna I; Laboratory of Radiation Biophysics and Radiobiology, Department of Physics, University of Pavia, Pavia, Italy.
  • Lonati L; Laboratory of Radiation Biophysics and Radiobiology, Department of Physics, University of Pavia, Pavia, Italy.
  • Lamartinière Y; Aix Marseille University, Avignon Université, CNRS, IRD, IMBE, Marseille, France.
  • Orsière T; Aix Marseille University, Avignon Université, CNRS, IRD, IMBE, Marseille, France.
  • Rousseau B; Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SCBM, F-91191, Gif-sur-Yvette, France.
  • Ottolenghi A; Laboratory of Radiation Biophysics and Radiobiology, Department of Physics, University of Pavia, Pavia, Italy.
  • Jha A; School of Biological and Marine Sciences, University of Plymouth, Plymouth, United Kingdom.
  • Lebaron-Jacobs L; Aix Marseille University, CEA, CNRS, BIAM, Saint Paul-Lez-Durance, France.
  • Grisolia C; CEA, IRFM, F-13108, Saint Paul lez Durance, France.
  • Malard V; Aix Marseille University, CEA, CNRS, BIAM, Saint Paul-Lez-Durance, France.
Radiat Res ; 195(3): 265-274, 2021 03 01.
Article em En | MEDLINE | ID: mdl-33400793
Tritium has been receiving worldwide attention, particularly because of its production and use in existing fission reactors and future nuclear fusion technologies, leading to an increased risk of release in the environment. Linking human health effects to low-dose tritium exposures presents a challenge for many reasons. Among these: biological effects strongly depend on the speciation of tritiated products and exposure pathway; large dosimetric uncertainties may exist; measurements using in vitro cell cultures generally lack a description of effects at the tissue level, while large-scale animal studies might be ethically questionable and too highly demanding in terms of resources. In this context, three-dimensional models of the human airway epithelium are a powerful tool to investigate potential toxicity induced upon inhalation of radioactive products in controlled physiological conditions. In this study we exposed such a model to tritiated water (HTO) for 24 h, with a range of activity levels (up to ∼33 kBq µl-1 cm-2). After the exposures, we measured cell viability, integrity of epithelial layer and pro-inflammatory response at different post-exposure time-points. We also quantified tritium absorption and performed dosimetric estimates considering HTO passage through the epithelial layer, leading to reconstructed upper limits for the dose to the tissue of less than 50 cGy cumulative dose for the highest activity. Upon exposure to the highest activity, cell viability was not decreased; however, we observed a small effect on epithelial integrity and an inflammatory response persisting after seven days. These results represent a reference condition and will guide future experiments using human airway epithelium to investigate the effects of other peculiar tritiated products.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Trítio / Água / Epitélio / Pulmão Limite: Animals / Humans Idioma: En Revista: Radiat Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Trítio / Água / Epitélio / Pulmão Limite: Animals / Humans Idioma: En Revista: Radiat Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Itália