fuPCR as diagnostic method for the detection of rare fungal pathogens, such as Trichosporon, Cryptococcus and Fusarium.

Scharf, Sebastian; Bartels, Anna; Kondakci, Mustafa; Haas, Rainer; Pfeffer, Klaus; Henrich, Birgit

Scharf, Sebastian; Bartels, Anna; Kondakci, Mustafa; Haas, Rainer; Pfeffer, Klaus; Henrich, Birgit.

Afiliação

Scharf S; Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
Bartels A; Department of Haematology, Oncology and Clinical Immunology, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
Kondakci M; Department of Haematology, Oncology and Clinical Immunology, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
Haas R; Department of Haematology, Oncology and Clinical Immunology, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
Pfeffer K; Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
Henrich B; Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.

Med Mycol ; 59(11): 1101-1113, 2021 Nov 03.

Article em En | MEDLINE | ID: mdl-34379780

We validated a set of PCR reactions (fuPCR) for use in routine diagnostic. In contrast to culture and serological methods, only by fuPCR pathogenic yeasts (Cryptococcus and Trichosporon) and molds (Aspergillus and Fusarium) were detected in respiratory rinses and blood of hematological patients.

Assuntos

Cryptococcus/isolamento & purificação; Fusarium/isolamento & purificação; Neoplasias Hematológicas/complicações; Micoses/diagnóstico; Micoses/etiologia; Reação em Cadeia da Polimerase em Tempo Real/métodos; Trichosporon/isolamento & purificação; Cryptococcus/genética; Técnicas e Procedimentos Diagnósticos; Feminino; Fusarium/genética; Voluntários Saudáveis; Humanos; Masculino; Micoses/genética; Trichosporon/genética

Palavras-chave

PCR; diagnostic; fungi; hematology; infection

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Trichosporon / Neoplasias Hematológicas / Cryptococcus / Reação em Cadeia da Polimerase em Tempo Real / Fusarium / Micoses Tipo de estudo: Diagnostic_studies / Risk_factors_studies / Screening_studies Limite: Female / Humans / Male Idioma: En Revista: Med Mycol Assunto da revista: MEDICINA VETERINARIA / MICROBIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Alemanha

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