Semi-rational design of L-amino acid deaminase for production of pyruvate and D-alanine by Escherichia coli whole-cell biocatalyst.

Liu, Ke; Yu, Haoran; Sun, Guoyun; Liu, Yanfeng; Li, Jianghua; Du, Guocheng; Lv, Xueqin; Liu, Long

Liu, Ke; Yu, Haoran; Sun, Guoyun; Liu, Yanfeng; Li, Jianghua; Du, Guocheng; Lv, Xueqin; Liu, Long.

Afiliação

Liu K; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Yu H; College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
Sun G; Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China.
Liu Y; College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
Li J; Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311200, China.
Du G; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Lv X; Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China.
Liu L; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.

Amino Acids ; 53(9): 1361-1371, 2021 Sep.

Article em En | MEDLINE | ID: mdl-34417892

Assuntos

Alanina/metabolismo; Amidoidrolases/metabolismo; Proteínas de Bactérias/metabolismo; Escherichia coli/metabolismo; Mutação; Proteus mirabilis/enzimologia; Ácido Pirúvico/metabolismo; Amidoidrolases/genética; Proteínas de Bactérias/genética; Biocatálise; Escherichia coli/genética; Mutagênese Sítio-Dirigida; Engenharia de Proteínas

Palavras-chave

Genome engineering; High-throughput screening; L-amino acid deaminase; Pyruvate; Semi-rational design; Whole-cell biocatalysis

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteus mirabilis / Proteínas de Bactérias / Ácido Pirúvico / Alanina / Escherichia coli / Amidoidrolases / Mutação Idioma: En Revista: Amino Acids Assunto da revista: BIOQUIMICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google