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Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion.
Junqueira Alves, Chrystian; Dariolli, Rafael; Haydak, Jonathan; Kang, Sangjo; Hannah, Theodore; Wiener, Robert J; DeFronzo, Stefanie; Tejero, Rut; Gusella, Gabriele L; Ramakrishnan, Aarthi; Alves Dias, Rodrigo; Wojcinski, Alexandre; Kesari, Santosh; Shen, Li; Sobie, Eric A; Rodrigues Furtado de Mendonça, José Paulo; Azeloglu, Evren U; Zou, Hongyan; Friedel, Roland H.
Afiliação
  • Junqueira Alves C; Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Dariolli R; Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Haydak J; Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Kang S; Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Hannah T; Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Wiener RJ; Division of Cardiology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • DeFronzo S; Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Tejero R; Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Gusella GL; Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Ramakrishnan A; Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Alves Dias R; Department of Physics, Federal University of Juiz de Fora, Juiz de Fora, Brazil.
  • Wojcinski A; Pacific Neuroscience Institute and John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica, CA, USA.
  • Kesari S; Pacific Neuroscience Institute and John Wayne Cancer Institute at Providence Saint John's Health Center, Santa Monica, CA, USA.
  • Shen L; Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Sobie EA; Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Rodrigues Furtado de Mendonça JP; Department of Physics, Federal University of Juiz de Fora, Juiz de Fora, Brazil.
  • Azeloglu EU; Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Zou H; Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Friedel RH; Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. hongyan.zou@mssm.edu.
Nat Commun ; 12(1): 6019, 2021 10 14.
Article em En | MEDLINE | ID: mdl-34650052
During morphogenesis, molecular mechanisms that orchestrate biomechanical dynamics across cells remain unclear. Here, we show a role of guidance receptor Plexin-B2 in organizing actomyosin network and adhesion complexes during multicellular development of human embryonic stem cells and neuroprogenitor cells. Plexin-B2 manipulations affect actomyosin contractility, leading to changes in cell stiffness and cytoskeletal tension, as well as cell-cell and cell-matrix adhesion. We have delineated the functional domains of Plexin-B2, RAP1/2 effectors, and the signaling association with ERK1/2, calcium activation, and YAP mechanosensor, thus providing a mechanistic link between Plexin-B2-mediated cytoskeletal tension and stem cell physiology. Plexin-B2-deficient stem cells exhibit premature lineage commitment, and a balanced level of Plexin-B2 activity is critical for maintaining cytoarchitectural integrity of the developing neuroepithelium, as modeled in cerebral organoids. Our studies thus establish a significant function of Plexin-B2 in orchestrating cytoskeletal tension and cell-cell/cell-matrix adhesion, therefore solidifying the importance of collective cell mechanics in governing stem cell physiology and tissue morphogenesis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Citoesqueleto / Actomiosina / Adesão Celular / Proteínas do Tecido Nervoso Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco / Citoesqueleto / Actomiosina / Adesão Celular / Proteínas do Tecido Nervoso Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos