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Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.
Stangherlin, Alessandra; Watson, Joseph L; Wong, David C S; Barbiero, Silvia; Zeng, Aiwei; Seinkmane, Estere; Chew, Sew Peak; Beale, Andrew D; Hayter, Edward A; Guna, Alina; Inglis, Alison J; Putker, Marrit; Bartolami, Eline; Matile, Stefan; Lequeux, Nicolas; Pons, Thomas; Day, Jason; van Ooijen, Gerben; Voorhees, Rebecca M; Bechtold, David A; Derivery, Emmanuel; Edgar, Rachel S; Newham, Peter; O'Neill, John S.
Afiliação
  • Stangherlin A; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Watson JL; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Wong DCS; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Barbiero S; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Zeng A; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Seinkmane E; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Chew SP; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Beale AD; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Hayter EA; Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
  • Guna A; UCSF, San Francisco, CA, USA.
  • Inglis AJ; California Institute of Technology, Pasadena, CA, USA.
  • Putker M; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Bartolami E; Crown Bioscience Netherlands B.V., Utrecht, The Netherlands.
  • Matile S; Department of Chemistry, University of Geneva, Geneva, Switzerland.
  • Lequeux N; CEA, IRIG, SyMMES, Grenoble, France.
  • Pons T; Department of Chemistry, University of Geneva, Geneva, Switzerland.
  • Day J; LPEM - ESPCI Paris, PSL, CNRS, Sorbonne Université, Paris, France.
  • van Ooijen G; LPEM - ESPCI Paris, PSL, CNRS, Sorbonne Université, Paris, France.
  • Voorhees RM; Department of Earth Sciences, University of Cambridge, Cambridge, UK.
  • Bechtold DA; School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
  • Derivery E; California Institute of Technology, Pasadena, CA, USA.
  • Edgar RS; Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
  • Newham P; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • O'Neill JS; Department of Infectious Diseases, Imperial College London, London, UK.
Nat Commun ; 12(1): 6035, 2021 10 15.
Article em En | MEDLINE | ID: mdl-34654800
Between 6-20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For cell viability, and to maintain volume within narrow limits, the daily variation in osmotic potential exerted by changes in the soluble proteome must be counterbalanced. The mechanisms and consequences of this osmotic compensation have not been investigated before. In cultured cells and in tissue we find that compensation involves electroneutral active transport of Na+, K+, and Cl- through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes confer daily variation in electrical activity. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osmose / Fenômenos Fisiológicos Celulares / Ritmo Circadiano / Transporte de Íons Limite: Animals Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Osmose / Fenômenos Fisiológicos Celulares / Ritmo Circadiano / Transporte de Íons Limite: Animals Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2021 Tipo de documento: Article