Synthesis of an Enzyme-Mediated Reversible Cross-linked Hydrogel for Cell Culture.
Biomacromolecules
; 22(12): 5118-5127, 2021 12 13.
Article
em En
| MEDLINE
| ID: mdl-34752066
ABSTRACT
Detachment of fragile cell types cultured on two-dimensional (2D) surfaces has been shown to be detrimental to their viability. For example, detachment of induced pluripotent stem cell (iPSC)-derived neurons grown in vitro in 2D typically results in loss of neuronal connections and/or cell death. Avoiding cell detachment altogether by changing the properties of the substrate on which the cells are grown is a compelling strategy to maintain cell viability. Here, we present the synthesis of a reversible cross-linked hydrogel that is sufficiently stable for cell culture and differentiation and is cleaved by an external stimulus, facilitating injection. Specifically, hyaluronan (HA) and methylcellulose (MC) were modified with ketone and aldehyde groups, respectively, and a TEV protease-degradable peptide was synthesized via solid-state synthesis and modified at both termini with oxyamine groups to cross-link HA-ketone and MC-aldehyde to produce oxime-cross-linked HA × MC. The HA × MC hydrogel demonstrated good stability, enzyme-sensitive degradation, and cytocompatibility with iPSC-derived neural progenitor cells, laying the framework for broad applicability.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes Induzidas
/
Células-Tronco Neurais
Idioma:
En
Revista:
Biomacromolecules
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Canadá