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SYBR® Green RT-qPCR for the Universal Detection of Citrus Viroids.
Vidalakis, Georgios; Wang, Jinbo; Dang, Tyler; Rucker, Tavia; Bodaghi, Sohrab; Tan, Shi-Hua; Lavagi-Craddock, Irene; Syed, Alexandra; Uribe, Gerardo; Hsieh, Yi; Carbajal-Moreno, Joana.
Afiliação
  • Vidalakis G; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA. vidalg@ucr.edu.
  • Wang J; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA. Jinbo.Wang@usda.gov.
  • Dang T; United States Department of Agriculture-APHIS-Biotechnology Regulatory Service-Biotechnology Risk Assessment Programs, Riverdale, MD, USA. Jinbo.Wang@usda.gov.
  • Rucker T; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
  • Bodaghi S; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
  • Tan SH; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
  • Lavagi-Craddock I; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
  • Syed A; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
  • Uribe G; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
  • Hsieh Y; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
  • Carbajal-Moreno J; Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA.
Methods Mol Biol ; 2316: 211-217, 2022.
Article em En | MEDLINE | ID: mdl-34845697
ABSTRACT
Quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR have now become the gold standard for molecular diagnostics because of its sensitivity, specificity, and reproducibility. In addition, qPCR diagnostics are flexible because they can be scaled for high- or low-throughput applications. Here we describe an optimized assay and workflow for the universal detection of eight citrus viroid species and their variants by RT-qPCR. The assay allows for quick and efficient molecular detection of viroids without the need to run RT-qPCR for each individual viroid species.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Viroides / Citrus Tipo de estudo: Diagnostic_studies Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Viroides / Citrus Tipo de estudo: Diagnostic_studies Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos