Macrophage calcium reporter mice reveal immune cell communication in vitro and in vivo.
Cell Rep Methods
; 1(8)2021 12 20.
Article
em En
| MEDLINE
| ID: mdl-35079727
ABSTRACT
Cell communication underlies emergent functions in diverse cell types and tissues. Recent evidence suggests that macrophages are organized in communicating networks, but new tools are needed to quantitatively characterize the resulting cellular conversations. Here, we infer cell communication from spatiotemporal correlations of intracellular calcium dynamics that are non-destructively imaged across cell populations expressing genetically encoded calcium indicators. We describe a hematopoietic calcium reporter mouse (Csf1rCreGCaMP5fl) and a computational analysis pipeline for inferring communication between reporter cells based on "excess synchrony." We observed signals suggestive of cell communication in macrophages treated with immune-stimulatory DNA in vitro and tumor-associated immune cells imaged in a dorsal window chamber model in vivo. Together, the methods described here expand the toolkit for discovery of cell communication events in macrophages and other immune cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cálcio da Dieta
/
Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Cell Rep Methods
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos