A water-soluble fluorescent probe for real-time visualization of γ-glutamyl transpeptidase activity in living cells.
Bioorg Med Chem Lett
; 68: 128762, 2022 07 15.
Article
em En
| MEDLINE
| ID: mdl-35490954
ABSTRACT
γ-glutamyl transpeptidase (GGT) is a kind of cell-surface enzyme that is overexpressed in many cancer cells. It is of great significance to develop an ideal tool for the diagnosis of GGT-rich cancer cells. Here, we reported a simple-structured but effective imaging probe for the detection of GGT activity. In the presence of GGT, the γ-glutamyl linkage could be cleaved specifically to produce amino-substituted product, resulting in significant fluorescence enhancement at 578 nm. Moreover, we successfully employed the probe to monitor GGT activity in HepG2 cells. We envisaged that such a simple but effective imaging tool could improve the practical applications for bioimaging.
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Base de dados:
MEDLINE
Assunto principal:
Corantes Fluorescentes
/
Gama-Glutamiltransferase
Limite:
Humans
Idioma:
En
Revista:
Bioorg Med Chem Lett
Assunto da revista:
BIOQUIMICA
/
QUIMICA
Ano de publicação:
2022
Tipo de documento:
Article