Protocol to isolate mature thymic T cell subsets using fluorescence-activated cell sorting for assessing gene expression by RNA-seq and transcription factor binding across the genome by CUT&RUN.

Theofilatos, Dimitris; Äijö, Tarmo; Tsagaratou, Ageliki

Protocol to isolate mature thymic T cell subsets using fluorescence-activated cell sorting for assessing gene expression by RNA-seq and transcription factor binding across the genome by CUT&RUN.

Theofilatos, Dimitris; Äijö, Tarmo; Tsagaratou, Ageliki.

Afiliação

Theofilatos D; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: dimitris_theofilatos@med.unc.edu.
Äijö T; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Tsagaratou A; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Microbiology, University of North Carolina, Chapel Hill, NC 27599, USA. Electronic address: ageliki_tsagaratou@med.unc.edu.

STAR Protoc ; 3(4): 101839, 2022 12 16.

Article em En | MEDLINE | ID: mdl-36386867

Assuntos

Subpopulações de Linfócitos T; Fatores de Transcrição; Camundongos; Animais; Citometria de Fluxo; RNA-Seq; Fatores de Transcrição/genética; Expressão Gênica

Palavras-chave

Cell isolation; ChIPseq; Flow Cytometry/Mass Cytometry; Gene Expression; Immunology; RNAseq

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Subpopulações de Linfócitos T Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2022 Tipo de documento: Article

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