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Glucose Infusion Induced Change in Intracellular pH and Its Relationship with Tumor Glycolysis in a C6 Rat Model of Glioblastoma.
Qi, Qi; Fox, Matthew S; Lim, Heeseung; Sullivan, Rebecca; Li, Alex; Bellyou, Miranda; Desjardins, Lise; McClennan, Andrew; Bartha, Robert; Hoffman, Lisa; Scholl, Timothy J; Lee, Ting-Yim; Thiessen, Jonathan D.
Afiliação
  • Qi Q; Department of Medical Biophysics, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Fox MS; Molecular Imaging Program, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Lim H; Department of Physics and Astronomy, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Sullivan R; Department of Physics and Astronomy, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Li A; Imaging Program, Lawson Health Research Institute, London, ON, N6A 4V2, Canada.
  • Bellyou M; Department of Medical Biophysics, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Desjardins L; Robarts Research Institute, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • McClennan A; Molecular Imaging Program, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Bartha R; Imaging Program, Lawson Health Research Institute, London, ON, N6A 4V2, Canada.
  • Hoffman L; Department of Pathology, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Scholl TJ; Robarts Research Institute, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Lee TY; Robarts Research Institute, The University of Western Ontario, London, ON, N6A 3K7, Canada.
  • Thiessen JD; Imaging Program, Lawson Health Research Institute, London, ON, N6A 4V2, Canada.
Mol Imaging Biol ; 25(2): 271-282, 2023 04.
Article em En | MEDLINE | ID: mdl-36418769
ABSTRACT

INTRODUCTION:

The reliance on glycolytic metabolism is a hallmark of tumor metabolism. Excess acid and protons are produced, leading to an acidic tumor environment. Therefore, we explored the relationship between the tumor glycolytic metabolism and tissue pH by comparing 18F-fluorodeoxyglucose positron emission tomography (FDG-PET) and hyperpolarized [1-13C]pyruvate MR spectroscopy imaging (MRSI) to chemical exchange saturation transfer (CEST) MRI measurements of tumor pH.

METHODS:

106 C6 glioma cells were implanted in the brains of male Wistar rats (N = 11) using stereotactic surgery. A 60-min PET acquisition after a bolus of FDG was performed at 11-13 days post implantation, and standardized uptake value (SUV) was calculated. CEST measurements were acquired the following day before and during constant infusion of glucose solution. Tumor intracellular pH (pHi) was evaluated using amine and amide concentration-independent detection (AACID) CEST MRI. The change of pHi (∆pHi) was calculated as the difference between pHi pre- and during glucose infusion. Rats were imaged immediately with hyperpolarized [1-13C]pyruvate MRSI. Regional maps of the ratio of LacPyr were acquired. The correlations between SUV, LacPyr ratio, and ∆pHi were evaluated using Pearson's correlation.

RESULTS:

A decrease of 0.14 in pHi was found after glucose infusion in tumor region. Significant correlations between tumor glycolysis measurements of LacPyr and ∆pHi within the tumor (ρ = 0.83, P = 0.01) and peritumoral region (ρ = 0.76, P = 0.028) were observed. No significant correlations were found between tumor SUV and ∆pHi within the tumor (ρ = - 0.45, P = 0.17) and peritumor regions (ρ = - 0.6, P = 0.051).

CONCLUSION:

AACID detected the changes in pHi induced by glucose infusion. Significant correlations between tumor glycolytic measurement of LacPyr and tumoral and peritumoral pHi and ∆pHi suggest the intrinsic relationship between tumor glycolytic metabolism and the tumor pH environment as well as the peritumor pH environment.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Encefálicas / Glioblastoma Limite: Animals Idioma: En Revista: Mol Imaging Biol Assunto da revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Encefálicas / Glioblastoma Limite: Animals Idioma: En Revista: Mol Imaging Biol Assunto da revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá