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Qualitative Analysis of Novel Flavonoid Adducts from Nerve Agent Tabun-Exposed Arabidopsis thaliana (L.) Based on Quadrupole-Time of Flight Mass Spectrometry.
Xing, Zhongfang; Zhang, Ruiqian; Zhao, Zhehui; Yuan, Ling; Yu, Huilan; Yang, Yang; Yang, Yuntao; Liu, Shilei; Pei, Chengxin.
Afiliação
  • Xing Z; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
  • Zhang R; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
  • Zhao Z; State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Department of Medicinal Chemistry, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
  • Yuan L; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
  • Yu H; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
  • Yang Y; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
  • Yang Y; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
  • Liu S; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
  • Pei C; State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
Molecules ; 28(6)2023 Mar 12.
Article em En | MEDLINE | ID: mdl-36985553
Flavonoids are a kind of secondary metabolite which widely exist in plants. They contain a lot of active hydroxyls, which can react with toxic chemicals to produce potential exposure biomarkers. In this article, the model plant Arabidopsis thaliana (L.) was exposed to the nerve agent O-Ethyl N,N-dimethyl phosphoramidocyanidate (Tabun). By comparing with the plant not exposed to Tabun, some characteristic ions were identified by quadrupole-time of flight mass spectrometry in the acetonitrile extract of the exposed leaves. These characteristic ions were selected as parent ions to produce product ion mass spectra (PIMS). Some interesting fragmentation pathways were revealed, including neutral loss of glucoside, rhamnose and ethylene. O-Ethyl N,N-dimethyl phosphoryl modified flavonoids were deduced from assignment of the PIMS. The element components and the accurate mass of the product ions from each parent ion matched well with those of the proposed fragmentation pathways. Through comparison with the PIMS of structurally closely related chemical of Isobutyl methylphosphonyl modified flavonoids, the structures and the fragmentation pathways of the O-Ethyl N,N-dimethyl phosphoryl modified flavonoids were finally confirmed. Successfully finding and identifying these three specific exposure biomarkers in plants provided a new strategy for the retrospective analysis of organophosphorus exposure and forensic analysis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Agentes Neurotóxicos Tipo de estudo: Observational_studies / Prognostic_studies / Qualitative_research Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Agentes Neurotóxicos Tipo de estudo: Observational_studies / Prognostic_studies / Qualitative_research Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China