Measuring Immune Cell Movement Toward the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay.

Mylod, Eimear; Lysaght, Joanne; Conroy, Melissa J

Mylod, Eimear; Lysaght, Joanne; Conroy, Melissa J.

Afiliação

Mylod E; Cancer Immunology and Immunotherapy Group, Department of Surgery, Trinity Translational Medicine Institute, St James's Hospital, Dublin, Ireland.
Lysaght J; Cancer Immunology and Immunotherapy Group, Department of Surgery, Trinity Translational Medicine Institute, St James's Hospital, Dublin, Ireland.
Conroy MJ; Cancer Immunology Research Group, Department of Physiology, School of Medicine, Trinity College Dublin, Dublin, Ireland. meconroy@tcd.ie.

Methods Mol Biol ; 2645: 231-240, 2023.

Article em En | MEDLINE | ID: mdl-37202623

ABSTRACT

ABSTRACT

Migration assays are used to measure cell movement toward a variety of chemoattractants in a controlled environment. Here we describe a method for a Boyden chamber-based migration assay using conditioned media generated from the tumor, liver, and visceral adipose tissue of cancer patients.

Assuntos

Fatores Quimiotáticos; Quimiotaxia; Humanos; Movimento Celular; Ensaios de Migração Celular/métodos; Linhagem Celular Tumoral; Meios de Cultivo Condicionados

Palavras-chave

Boyden chamber; Cell migration; Chemokines; Chemotaxis; Immune cells

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores Quimiotáticos / Quimiotaxia Limite: Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Irlanda

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