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Structure-Driven Liquid Microjunction Surface-Sampling Probe Mass Spectrometry.
Kertesz, Vilmos; Khalid, Muneeba; Retterer, Scott T; Cahill, John F.
Afiliação
  • Kertesz V; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6131, United States.
  • Khalid M; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6131, United States.
  • Retterer ST; Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6131, United States.
  • Cahill JF; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6131, United States.
Anal Chem ; 95(39): 14521-14525, 2023 10 03.
Article em En | MEDLINE | ID: mdl-37738474
ABSTRACT
The rhizosphere is the narrow region of soil surrounding the roots of plants that is influenced by root exudates, root secretions, and associated microbial communities. This region is crucial to plant growth and development and plays a critical role in nutrient uptake, disease resistance, and soil transformation. Understanding the function of exogenous compounds in the rhizosphere starts with determining the spatiotemporal distribution of these molecular components. Using liquid microjunction surface-sampling probe mass spectrometry (LMJ-SSP-MS) and microfluidic devices with attached microporous membranes enables in situ, nondisruptive, and nondestructive spatiotemporal measurement of exogenous compounds from plant roots. However, long imaging times (>2 h) can negatively affect plant heath and limit temporal studies. Here, we present a novel strategy to optimize the number and location of sampling sites on these microporous membrane-covered microfluidic devices. This novel, "structure-driven" sampling workflow takes into consideration the channel structure of the microfluidic device to maximize sampling from the channels and minimize acquisition time (∼4× less time in some cases while providing similar chemical image accuracy), thus reducing stress on plants during in situ LMJ-SSP-MS analysis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Solo / Microbiota Idioma: En Revista: Anal Chem Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Solo / Microbiota Idioma: En Revista: Anal Chem Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos