Nanofibrous Membrane Promotes and Sustains Vascular Endothelial Barrier Function.
ACS Appl Bio Mater
; 6(11): 4988-4997, 2023 11 20.
Article
em En
| MEDLINE
| ID: mdl-37862245
ABSTRACT
The vascular endothelium serves as a physical barrier between the circulating blood and surrounding tissue and acts as a critical regulator of various physiological processes. In vitro models involving vasculature rely on the maintenance of the endothelial barrier function. In this study, we fabricated 2D aligned nanofibrous membranes with distinct pore sizes via electrospinning and investigated the effect of membrane pore size on endothelial barrier function. Our results demonstrated that the use of the nanofibrous membranes promoted the formation of a tight vascular endothelium and sustained barrier function for over one month in comparison with conventional transwell setups. Moreover, the examination of the nucleocytoplasmic localization of yes-associated protein (YAP) in the endothelial cells indicated that nanofibrous membrane promoted YAP expression and its nuclear localization, critical to endothelial barrier function. Furthermore, the comparison of permeability between random and aligned nanofibrous membranes underscored the importance of pore size in preserving barrier function. Our findings offer a valuable strategy for creating more physiologically relevant in vitro vascular models and contribute to the understanding of endothelial barrier formation and maintenance mechanisms.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Endoteliais
/
Nanofibras
Idioma:
En
Revista:
ACS Appl Bio Mater
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos