Primed conversion: The emerging player of precise and nontoxic photoconversion.
J Microsc
; 2023 Nov 08.
Article
em En
| MEDLINE
| ID: mdl-37937409
ABSTRACT
In 2015, we reported primed conversion, a novel way to convert green-to-red photoconvertible fluorescent proteins, which emerges as a powerful tool for precision optical imaging. Primed conversion uses the intercept of blue and red-to-far-red light instead of traditional violet or near-UV light illumination which offers a series of advantages. Here, we review the fundamental principles and applications of primed conversion with a focus on its use in single-cell labelling and lineage tracing. We provide a historical perspective of lineage tracing techniques, thereby covering basic principles of fluorescence, photoconvertible fluorescent proteins, and eventually primed conversion. We then present the molecular requirements for primed conversion to take place and showcase how it can be used for dual-colour high-fidelity lineage tracing. Further, we discuss potential future developments of the primed conversion imaging toolkit that can benefit the study of both development and disease progression.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
J Microsc
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Reino Unido