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Poly-ß-hydroxybutyrate production by Synechocystis MT_a24 in a raceway pond using urban wastewater.
Grivalský, Tomás; Lakatos, Gergely Erno; Sterbová, Karolína; Manoel, João Artur Câmara; Belosa, Romana; Divoká, Petra; Kopp, Julian; Kriechbaum, Ricarda; Spadiut, Oliver; Zwirzitz, Alexander; Trenzinger, Kevin; Masojídek, Jirí.
Afiliação
  • Grivalský T; Institute of Microbiology of the Czech Academy of Sciences, Centre Algatech, Laboratory of Algal Biotechnology, Novohradská 237, Trebon, Czech Republic. grivalsky@alga.cz.
  • Lakatos GE; Institute of Microbiology of the Czech Academy of Sciences, Centre Algatech, Laboratory of Algal Biotechnology, Novohradská 237, Trebon, Czech Republic.
  • Sterbová K; Institute of Microbiology of the Czech Academy of Sciences, Centre Algatech, Laboratory of Algal Biotechnology, Novohradská 237, Trebon, Czech Republic.
  • Manoel JAC; Institute of Microbiology of the Czech Academy of Sciences, Centre Algatech, Laboratory of Algal Biotechnology, Novohradská 237, Trebon, Czech Republic.
  • Belosa R; Faculty of Science, University of South Bohemia, Branisovská 1645/31a, Ceské Budejovice, Czech Republic.
  • Divoká P; Institute of Microbiology of the Czech Academy of Sciences, Centre Algatech, Laboratory of Algal Biotechnology, Novohradská 237, Trebon, Czech Republic.
  • Kopp J; Institute of Microbiology of the Czech Academy of Sciences, Centre Algatech, Laboratory of Algal Biotechnology, Novohradská 237, Trebon, Czech Republic.
  • Kriechbaum R; Technische Universität Wien, Institute of Chemical, Environmental and Bioscience Engineering, Research Division Biochemical Engineering, Getreidemarkt 9, Vienna, Austria.
  • Spadiut O; Technische Universität Wien, Institute of Chemical, Environmental and Bioscience Engineering, Research Division Biochemical Engineering, Getreidemarkt 9, Vienna, Austria.
  • Zwirzitz A; Technische Universität Wien, Institute of Chemical, Environmental and Bioscience Engineering, Research Division Biochemical Engineering, Getreidemarkt 9, Vienna, Austria.
  • Trenzinger K; Biosciences Research Group, University of Applied Sciences, Stelzhamerstraße 23, Wels, Austria.
  • Masojídek J; Biosciences Research Group, University of Applied Sciences, Stelzhamerstraße 23, Wels, Austria.
Appl Microbiol Biotechnol ; 108(1): 44, 2024 Dec.
Article em En | MEDLINE | ID: mdl-38180554
ABSTRACT
Poly-ß-hydroxybutyrate (PHB) is a potential source of biodegradable plastics that are environmentally friendly due to their complete degradation to water and carbon dioxide. This study aimed to investigate PHB production in the cyanobacterium Synechocystis sp. PCC6714 MT_a24 in an outdoor bioreactor using urban wastewater as a sole nutrient source. The culture was grown in a thin-layer raceway pond with a working volume of 100 L, reaching a biomass density of up to 3.5 g L-1 of cell dry weight (CDW). The maximum PHB content was found under nutrient-limiting conditions in the late stationary phase, reaching 23.7 ± 2.2% PHB per CDW. These data are one of the highest reported for photosynthetic production of PHB by cyanobacteria, moreover using urban wastewater in pilot-scale cultivation which multiplies the potential of sustainable cultivation approaches. Contamination by grazers (Poterioochromonas malhamensis) was managed by culturing Synechocystis in a highly alkaline environment (pH about 10.5) which did not significantly affect the culture growth. Furthermore, the strain MT_a24 showed significant wastewater nutrient remediation removing about 72% of nitrogen and 67% of phosphorus. These trials demonstrate that the photosynthetic production of PHB by Synechocystis sp. PCC6714 MT_a24 in the outdoor thin-layer bioreactor using urban wastewater and ambient carbon dioxide. It shows a promising approach for the cost-effective and sustainable production of biodegradable carbon-negative plastics. KEY POINTS • High PHB production by cyanobacteria in outdoor raceway pond • Urban wastewater used as a sole source of nutrients for phototrophic growth • Potential for cost-effective and sustainable production of biodegradable plastics.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Synechocystis / Plásticos Biodegradáveis Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: República Tcheca

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Synechocystis / Plásticos Biodegradáveis Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: República Tcheca