Butorphanol inhibits androgen biosynthesis and metabolism in rat immature Leydig cells in vitro.
Can J Physiol Pharmacol
; 102(4): 270-280, 2024 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-38258745
ABSTRACT
Butorphanol is a synthetic opioid analgesic medication that is primarily used for the management of pain. Butorphanol may have an inhibitory effect on androgen biosynthesis and metabolism in rat immature Leydig cells. The objective of this study was to investigate the influence of butorphanol on androgen secretion by rat Leydig cells isolated from the 35-day-old male rats. Rat Leydig cells were cultured with 0.5-50 µM butorphanol for 3 h in vitro. Butorphanol at 5 and 50 µM significantly inhibited androgen secretion in immature Leydig cells. At 50 µM, butorphanol also blocked the effects of luteinizing hormone (LH) and 8bromo-cAMP-stimulated androgen secretion and 22R-hydroxycholesterol- and pregnenolone-mediated androgen production. Further analysis of the results showed that butorphanol downregulated the expression of genes involved in androgen production, including Lhcgr (LH receptor), Cyp11a1 (cholesterol side-chain cleavage enzyme), Srd5a1 (5α-reductase 1), and Akr1c14 (3α-hydroxysteroid dehydrogenase). Additionally, butorphanol directly inhibited HSD3B1 (3ß-hydroxysteroid dehydrogenase 1) and SRD5A1 activity. In conclusion, butorphanol may have side effects of inhibiting androgen biosynthesis and metabolism in Leydig cells.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Androgênios
/
Células Intersticiais do Testículo
Limite:
Animals
Idioma:
En
Revista:
Can J Physiol Pharmacol
/
Can. j. physiol. pharmacol
/
Canadian journal of physiology and pharmacology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China