Rapidly mining candidate cotton drought resistance genes based on key indicators of drought resistance.
BMC Plant Biol
; 24(1): 129, 2024 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-38383284
ABSTRACT
BACKGROUND:
Focusing on key indicators of drought resistance is highly important for quickly mining candidate genes related to drought resistance in cotton.RESULTS:
In the present study, drought resistance was identified in drought resistance-related RIL populations during the flowering and boll stages, and multiple traits were evaluated; these traits included three key indicators plant height (PH), single boll weight (SBW) and transpiration rate (Tr). Based on these three key indicators, three groups of extreme mixing pools were constructed for BSA-seq. Based on the mapping interval of each trait, a total of 6.27 Mb QTL intervals were selected on chromosomes A13 (3.2 Mb), A10 (2.45 Mb) and A07 (0.62 Mb) as the focus of this study. Based on the annotation information and qRTâPCR analysis, three key genes that may be involved in the drought stress response of cotton were screened GhF6'H1, Gh3AT1 and GhPER55. qRTâPCR analysis of parental and extreme germplasm materials revealed that the expression of these genes changed significantly under drought stress. Cotton VIGS experiments verified the important impact of key genes on cotton drought resistance.CONCLUSIONS:
This study focused on the key indicators of drought resistance, laying the foundation for the rapid mining of drought-resistant candidate genes in cotton and providing genetic resources for directed molecular breeding of drought resistance in cotton.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Locos de Características Quantitativas
/
Resistência à Seca
Idioma:
En
Revista:
BMC Plant Biol
Assunto da revista:
BOTANICA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China