N6-methyltubercidin gives sterile cure in a cutaneous Leishmania amazonensis mouse model.
Parasitology
; 151(5): 506-513, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38533610
ABSTRACT
Leishmania is a trypanosomatid parasite that causes skin lesions in its cutaneous form. Current therapies rely on old and expensive drugs, against which the parasites have acquired considerable resistance. Trypanosomatids are unable to synthesize purines relying on salvaging from the host, and nucleoside analogues have emerged as attractive antiparasitic drug candidates. 4-Methyl-7-ß-D-ribofuranosyl-7H-pyrrolo[2,3-d]pyrimidine (CL5564), an analogue of tubercidin in which the amine has been replaced by a methyl group, demonstrates activity against Trypanosoma cruzi and Leishmania infantum. Herein, we investigated its in vitro and in vivo activity against L. amazonensis. CL5564 was 6.5-fold (P = 0.0002) more potent than milteforan™ (ML) against intracellular forms in peritoneal mouse macrophages, and highly selective, while combination with ML gave an additive effect. These results stimulated us to study the activity of CL5564 in mouse model of cutaneous Leishmania infection. BALB/c female and male mice infected by L. amazonensis treated with CL5564 (10 mg kg−1, intralesional route for five days) presented a >93% reduction of paw lesion size likely ML given orally at 40 mg kg−1, while the combination (10 + 40 mg kg−1 of CL5564 and ML, respectively) caused >96% reduction. The qPCR confirmed the suppression of parasite load, but only the combination approach reached 66% of parasitological cure. These results support additional studies with nucleoside derivatives.
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Base de dados:
MEDLINE
Assunto principal:
Leishmania mexicana
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Leishmaniose Cutânea
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Modelos Animais de Doenças
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Camundongos Endogâmicos BALB C
Limite:
Animals
Idioma:
En
Revista:
Parasitology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Brasil