CDK activity at the centrosome regulates the cell cycle.
Cell Rep
; 43(4): 114066, 2024 Apr 23.
Article
em En
| MEDLINE
| ID: mdl-38578823
ABSTRACT
In human cells and yeast, an intact "hydrophobic patch" substrate docking site is needed for mitotic cyclin centrosomal localization. A hydrophobic patch mutant (HPM) of the fission yeast mitotic cyclin Cdc13 cannot enter mitosis, but whether this is due to defective centrosomal localization or defective cyclin-substrate docking more widely is unknown. Here, we show that artificially restoring Cdc13-HPM centrosomal localization promotes mitotic entry and increases CDK (cyclin-dependent kinase) substrate phosphorylation at the centrosome and in the cytoplasm. We also show that the S-phase B-cyclin hydrophobic patch is required for centrosomal localization but not for S phase. We propose that the hydrophobic patch is essential for mitosis due to its requirement for the local concentration of cyclin-CDK with CDK substrates and regulators at the centrosome. Our findings emphasize the central importance of the centrosome as a hub coordinating cell-cycle control and explain why the cyclin hydrophobic patch is essential for mitosis.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
/
Ciclo Celular
/
Quinases Ciclina-Dependentes
/
Centrossomo
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Ciclina B
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Proteínas de Schizosaccharomyces pombe
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Mitose
Limite:
Humans
Idioma:
En
Revista:
Cell Rep
Ano de publicação:
2024
Tipo de documento:
Article