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The genetic regulatory architecture and epigenomic basis for age-related changes in rattlesnake venom.
Hogan, Michael P; Holding, Matthew L; Nystrom, Gunnar S; Colston, Timothy J; Bartlett, Daniel A; Mason, Andrew J; Ellsworth, Schyler A; Rautsaw, Rhett M; Lawrence, Kylie C; Strickland, Jason L; He, Bing; Fraser, Peter; Margres, Mark J; Gilbert, David M; Gibbs, H Lisle; Parkinson, Christopher L; Rokyta, Darin R.
Afiliação
  • Hogan MP; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
  • Holding ML; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
  • Nystrom GS; Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109.
  • Colston TJ; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
  • Bartlett DA; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
  • Mason AJ; Department of Biology, University of Puerto Rico at Mayagüez, Mayagüez, PR 00681.
  • Ellsworth SA; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
  • Rautsaw RM; Department of Biological Sciences, Clemson University, Clemson, SC 29634.
  • Lawrence KC; Department of Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210.
  • Strickland JL; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
  • He B; Department of Biological Sciences, Clemson University, Clemson, SC 29634.
  • Fraser P; Department of Integrative Biology, University of South Florida, Tampa, FL 33620.
  • Margres MJ; School of Biological Sciences, Washington State University, Pullman, WA 99164.
  • Gilbert DM; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
  • Gibbs HL; Department of Biological Sciences, Clemson University, Clemson, SC 29634.
  • Parkinson CL; Department of Biology, University of South Alabama, Mobile, AL 36688.
  • Rokyta DR; Department of Biological Science, Florida State University, Tallahassee, FL 32306.
Proc Natl Acad Sci U S A ; 121(16): e2313440121, 2024 Apr 16.
Article em En | MEDLINE | ID: mdl-38578985
ABSTRACT
Developmental phenotypic changes can evolve under selection imposed by age- and size-related ecological differences. Many of these changes occur through programmed alterations to gene expression patterns, but the molecular mechanisms and gene-regulatory networks underlying these adaptive changes remain poorly understood. Many venomous snakes, including the eastern diamondback rattlesnake (Crotalus adamanteus), undergo correlated changes in diet and venom expression as snakes grow larger with age, providing models for identifying mechanisms of timed expression changes that underlie adaptive life history traits. By combining a highly contiguous, chromosome-level genome assembly with measures of expression, chromatin accessibility, and histone modifications, we identified cis-regulatory elements and trans-regulatory factors controlling venom ontogeny in the venom glands of C. adamanteus. Ontogenetic expression changes were significantly correlated with epigenomic changes within genes, immediately adjacent to genes (e.g., promoters), and more distant from genes (e.g., enhancers). We identified 37 candidate transcription factors (TFs), with the vast majority being up-regulated in adults. The ontogenetic change is largely driven by an increase in the expression of TFs associated with growth signaling, transcriptional activation, and circadian rhythm/biological timing systems in adults with corresponding epigenomic changes near the differentially expressed venom genes. However, both expression activation and repression contributed to the composition of both adult and juvenile venoms, demonstrating the complexity and potential evolvability of gene regulation for this trait. Overall, given that age-based trait variation is common across the tree of life, we provide a framework for understanding gene-regulatory-network-driven life-history evolution more broadly.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Venenos de Crotalídeos / Serpentes Peçonhentas Limite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Venenos de Crotalídeos / Serpentes Peçonhentas Limite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2024 Tipo de documento: Article