Herbal-Based Green Synthesis of TB-ZnO-TiO(II) Nanoparticles Composite From Terminalia bellirica: Characterization, Toxicity Assay, Antioxidant Assay, and Antimicrobial Activity.

ABSTRACT

Background Terminalia bellirica leaf extract was used as an herbal to get an aqueous extract of Tb-ZnO-TiO2 (zinc and titanium dioxide) nanoparticles composite, and this was subsequently subjected to an analysis of its antioxidant properties and possible antimicrobial activity against gram-negative and gram-positive bacteria. Employing the 2,2-Diphenyl-1-picrylhydrazyl and hydrogen peroxide assay techniques for antioxidant properties. In addition to their biocompatibility, rapid biodegradability, and low toxicity, herbal-based nanoparticles (Tb-ZnO-TiO2 NPs composite) synthesized by T. bellirica have drawn a lot of interest as promising options for administering drugs and effective antimicrobial applications. Materials and methods The form and dimensions of the dispersion of the synthesized nanoparticles were investigated through scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy, and UV-visible for particle characterization. Nanoparticles were analyzed for antimicrobial activity using the well diffusion method. Ascorbic acid and vitamin E were used as two separate controls for antioxidant assay with different concentrations, and also toxicity assay was done by using zebrafish embryos. Results Tb-ZnO-TiO2 NPs composite were obtained as a powder, the X-beam diffraction (XRD) result revealed a small quantity of impurities and revealed that the structure was spherical in nature. A unique absorption peak for Tb-ZnO-TiO2 NPs composite may be seen in UV-Vis spectroscopy which is in the region of 260 to 320 nm. The Tb-ZnO-TiO2 NPs composite antibacterial efficacy was evaluated and showed noted antibacterial activity and free radical scavenging activity with less toxicity. Conclusion The results demonstrated the Tb-ZnO-TiO2 NPs composite has strong antioxidant qualities and enormous antibacterial activity obtained from T. bellirica extract. Therefore, the Tb-ZnO-TiO2 NPs composite synthesized nanoparticles can be used in biomedical applications as an effective antioxidant and antibacterial reagent.