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RNA Isolation from Ctenophores.
Kohn, Andrea B; Bobkova, Yelena; Moroz, Leonid L.
Afiliação
  • Kohn AB; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA.
  • Bobkova Y; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA.
  • Moroz LL; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA. moroz@whitney.ufl.edu.
Methods Mol Biol ; 2757: 201-214, 2024.
Article em En | MEDLINE | ID: mdl-38668968
ABSTRACT
RNA-seq or transcriptome analysis of individual cells and small cell populations is essential for virtually any biomedical field. Here, we examine and discuss the different methods of RNA isolation specific to ctenophores. We present a convenient, inexpensive, and reproducible protocol for RNA-seq libraries that are designed for low quantities of samples. We demonstrated these methods on early (one, two, four, eight cells) embryonic and developmental stages, tissues, and even a single aboral organ from the ctenophore Pleurobrachia bachei and other ctenophore species (e.g., Mnemiopsis, Bolinopsis, and Beroe).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Ctenóforos Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Ctenóforos Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos