Fecal shedding of Clostridioides difficile in calves in Sao Paulo state, Brazil.
Anaerobe
; 88: 102861, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38729514
ABSTRACT
OBJECTIVE:
This study aimed to evaluate the fecal shedding of C. difficile in calves on farms in Sao Paulo State, Brazil. MATERIALS ANDMETHODS:
Fecal samples (n = 300) were collected from diarrheic (n = 78) and nondiarrheic (n = 222) calves less than 60 days of age from 20 farms. Fecal samples were inoculated into enrichment broth supplemented with taurocholate and cultured under anaerobic conditions. Colonies suspected to be C. difficile were harvested for DNA extraction and then multiplex PCR for the detection of genes encoding toxins A and B and binary toxins. All toxigenic isolates were ribotyped and tested for antimicrobial susceptibility, and five selected strains were subjected to whole-genome sequencing to determine their sequence type. RESULTS ANDDISCUSSION:
C. difficile was isolated from 29.3 % (88/300) of the samples. All toxigenic isolates (17/88, 19.3 %) were classified as ribotypes RT046 (13/17-79.47 %, A+B+ CDT-) and RT126 (4/17 = 20.53 %, A+B+ CDT+). The sequenced strains from RT046 were classified as ST35 (Clade 1), while those from RT126 were classified as ST11 (Clade 5). No associations between the epidemiological factors in any of the groups and C. difficile isolation were observed. Most of the toxigenic isolates (16/17 = 94.41 %) were classified as multidrug-resistant. Calves can be an important source of toxigenic C. difficile strains, including multidrug-resistant isolates from ribotypes commonly observed in humans.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Bovinos
/
Clostridioides difficile
/
Infecções por Clostridium
/
Ribotipagem
/
Fezes
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Anaerobe
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Brasil