Evaluation of assays for nucleic acid testing for the prevention of chikungunya and dengue virus transmission by blood transfusion.
Transfusion
; 64(8): 1503-1508, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38877832
ABSTRACT
BACKGROUND:
The large dengue (DENV) and chikungunya (CHIKV) outbreaks observed during the last decade across the world, as well as local transmissions in non-endemic areas are a growing concern for blood safety. The aim of this study was to evaluate and compare the sensitivity of nucleic acid tests (NAT) detecting DENV and CHIKV RNA. MATERIALS ANDMETHODS:
Using DENV 1 to 4 International Standards, the limits of detection (LODs) calculated by probit analysis of two NAT assays; the cobas CHIKV/DENV assay (Roche Diagnostics) and the Procleix Dengue Virus Assay (Grifols) were compared. In addition, CHIKV-RNA LOD of the cobas CHIKV/DENV assay was evaluated.RESULTS:
For dengue, the 95% LOD of the cobas assay ranged between 4.10 [CI95% 2.70-8.19] IU/mL (DENV-2) and 7.07 [CI95% 4.34-14.89] IU/mL (DENV-4), and between 2.19 [CI95% 1.53-3.83] IU/mL (DENV-3) and 5.84 [CI95% 3.84-10.77] IU/mL (DENV-1) for Procleix assay. The Procleix assay had a significant lower LOD for DENV-3 (2.19 vs. 5.89 IU/mL) when compared to the cobas assay (p = 0.005). The 95% LOD for CHIKV-RNA detection of the cobas assay was 4.76 [CI95% 3.08-8.94] IU/mL.DISCUSSION:
The two NAT assays developed for blood donor screening evaluated in this study demonstrated high and similar analytical performance. Subject to an appropriate risk-benefit assessment, they can be used to support blood safety during outbreaks in endemic areas or in non-endemic areas as an alternative to deferring blood donors during local transmission likely to affect the blood supply. The development of multiplex assays is expected to optimize laboratory organization.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
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Vírus Chikungunya
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Dengue
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Vírus da Dengue
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Febre de Chikungunya
Limite:
Humans
Idioma:
En
Revista:
Transfusion
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
França