Transcriptomic Analysis of Pubertal and Adult Virgin Mouse Mammary Epithelial and Stromal Cell Populations.
J Mammary Gland Biol Neoplasia
; 29(1): 13, 2024 Jun 25.
Article
em En
| MEDLINE
| ID: mdl-38916673
ABSTRACT
Conflicting data exist as to how mammary epithelial cell proliferation changes during the reproductive cycle. To study the effect of endogenous hormone fluctuations on gene expression in the mouse mammary gland, we performed bulk RNAseq analyses of epithelial and stromal cell populations that were isolated either during puberty or at different stages of the adult virgin estrous cycle. Our data confirm prior findings that proliferative changes do not occur in every mouse in every cycle. We also show that during the estrous cycle the main gene expression changes occur in adipocytes and fibroblasts. Finally, we present a comprehensive overview of the Wnt gene expression landscape in different mammary gland cell types in pubertal and adult mice. This work contributes to understanding the effects of physiological hormone fluctuations and locally produced signaling molecules on gene expression changes in the mammary gland during the reproductive cycle and should be a useful resource for future studies investigating gene expression patterns in different cell types across different developmental timepoints.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Maturidade Sexual
/
Células Estromais
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Perfilação da Expressão Gênica
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Células Epiteliais
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Transcriptoma
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Glândulas Mamárias Animais
Limite:
Animals
Idioma:
En
Revista:
J Mammary Gland Biol Neoplasia
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Holanda