Low temperature inhibits food intake via TRPA1 channel activation in Nile tilapia (Oreochromis niloticus).
Mol Cell Endocrinol
; 592: 112333, 2024 Jul 22.
Article
em En
| MEDLINE
| ID: mdl-39048029
ABSTRACT
Low temperatures significantly influence feeding behavior in ectothermic vertebrates, but the underlying mechanisms remain elusive. This study investigated the role of transient receptor potential ankyrin 1 (TRPA1) channels in mediating the appetite-suppressing effects of low temperature in Nile tilapia. TRPA1 was found to be highly expressed in the hypothalamus and co-localized with neuropeptide Y (NPY) neurons. Exposure to low temperatures reduced feeding frequency and increased TRPA1 expression. In vitro experiments demonstrated that low temperature and TRPA1 agonists induced calcium influx, which was blocked by a TRPA1 inhibitor. TRPA1 expression exhibited post-prandial increases and was downregulated by fasting. TRPA1 activation dose-dependently inhibited food intake, while its inhibition restored feeding suppressed by low temperature. TRPA1 activation downregulated orexigenic factors and upregulated anorexigenic factors through Ca2+/calmodulin-dependent pathways. These findings suggest that TRPA1 plays a crucial role in sensing low temperatures and regulating feeding behavior in tilapia.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Mol Cell Endocrinol
Ano de publicação:
2024
Tipo de documento:
Article