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Bioflavonoid Baicalein Modulates Tetracycline Resistance by Inhibiting Efflux Pump in Staphylococcus aureus.
Moulick, Soumitra; Roy, Dijendra Nath.
Afiliação
  • Moulick S; TCG Lifesciences Private Limited, Kolkata, India.
  • Roy DN; Department of Biotechnology, National Institute of Technology Raipur, Chhattisgarh, India.
Microb Drug Resist ; 30(9): 363-371, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39133125
ABSTRACT
The rise in antibiotic resistance among bacterial pathogens, particularly Staphylococcus aureus, has become a critical global health issue, necessitating the search for novel antimicrobial agents. S. aureus uses various mechanisms to resist antibiotics, including the activation of efflux pumps, biofilm formation, and enzymatic modification of drugs. This study explores the potential of baicalein, a bioflavonoid from Scutellaria baicalensis, in modulating tetracycline resistance in S. aureus by inhibiting efflux pumps. The synergistic action of baicalein and tetracycline was evaluated through various assays. The minimum inhibitory concentration (MIC) of baicalein and tetracycline against S. aureus was 256 and 1.0 µg/mL, respectively. Baicalein at 64 µg/mL reduced the MIC of tetracycline by eightfold, indicating a synergistic effect (fractional inhibitory concentration index 0.375). Time-kill kinetics demonstrated a 1.0 log CFU/mL reduction in bacterial count after 24 hours with the combination treatment. The ethidium bromide accumulation assay showed that baicalein mediated significant inhibition of efflux pumps, with a dose-dependent increase in fluorescence. In addition, baicalein inhibited DNA synthesis by 73% alone and 92% in combination with tetracycline. It also markedly reduced biofilm formation and the invasiveness of S. aureus into HeLa cells by 52% at 64 µg/mL. These findings suggest that baicalein enhances tetracycline efficacy and could be a promising adjunct therapy to combat multidrug-resistant S. aureus infections.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Tetraciclina / Testes de Sensibilidade Microbiana / Biofilmes / Flavanonas / Sinergismo Farmacológico / Antibacterianos Limite: Humans Idioma: En Revista: Microb Drug Resist Assunto da revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Staphylococcus aureus / Tetraciclina / Testes de Sensibilidade Microbiana / Biofilmes / Flavanonas / Sinergismo Farmacológico / Antibacterianos Limite: Humans Idioma: En Revista: Microb Drug Resist Assunto da revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Índia