Comparison of Ins(1,4,5)P3 receptors from rat cerebellum and bovine adrenal cortex.
Biochim Biophys Acta
; 1175(3): 307-11, 1993 Feb 17.
Article
em En
| MEDLINE
| ID: mdl-8382083
ABSTRACT
Ins(1,4,5)P3 receptors in adrenal cortical and cerebellar membranes can be distinguished by their affinities for Ins(1,4,5)P3 as well as the potencies with which heparin and Mg2+ inhibit binding. We have found that the differences in Ins(1,4,5)P3 affinity and heparin inhibition are maintained upon receptor solubilization and purification. In contrast to this, heparin-agarose affinity purification of solubilized cerebellar receptors reduces the potency of Mg2+ inhibition to that in adrenal cortex. These results suggest that Ins(1,4,5)P3 receptors in adrenal cortex are structurally distinct from those in cerebellum. Monoclonal antibodies raised against C- and N-terminal regions of mouse cerebellar Ins(1,4,5)P3 receptors recognize 250-300-kDa proteins in both rat cerebellum and bovine adrenal cortex.
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Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Canais de Cálcio
/
Inositol 1,4,5-Trifosfato
/
Córtex Suprarrenal
/
Receptores Citoplasmáticos e Nucleares
/
Receptores de Superfície Celular
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Reino Unido