Identification of local camel populations in Turkiye using morphological, genetic, and breeding info.

The aim of this study was to investigate the demographic, morphological, and genetic characteristics of local camel populations reared in the Turkiye provinces of Aydin, Denizli, and Antalya, which have a long history of camel breeding. Although Turkiye has an old history of camel breeding in its historical process, the number of scientific studies aimed at identifying camel populations in Turkiye is almost negligible. In this study, local camel populations in Aydin, Denizli, and Antalya cities of Turkiye were examined in three dimensions as demographic, morphological, and genetic. A face-to-face survey of 117 breeders was used to determine demographic definitions. While the region where local camels were detected the most was determined as Antalya Region with 78.6%, it was determined that 82.6% of the breeders participating in the survey preferred to breed camels due to their docile temperament. Body measurements were made on 45 camels for morphological identification. Moreover, DNA were sampled with oral swabs from 57 camels for phylogenetic analyses using 16 SSR microsatellite loci to identify the genetic structure of local camel populations. The genetic analyses using SSR markers revealed that the camel populations in the Antalya region had a considerably more isolated genetic structure than the Aydin and Denizli populations, and consequently, these populations may be regarded the native camel population.

A practical, low-cost, short-term storage method for genomic DNA.

The aim of this study was to assess the DNA preservation capability of cellulose paper towel and blotting paper as low-cost alternatives to commercial DNA preservation products. Chicken blood was applied as DNA source to each paper towel, blotting paper, FTA® cards and DNA/RNA Shield™. All samples were stored at room temperature for 130 days. DNA extraction from dried blood spots was performed after various time periods using Tris-EDTA and NaOH protocols. PCR activity and the mean amount of DNA isolated from paper towels were reliable. The results of this study demonstrated that cellulose-based blotting paper and especially paper towel had considerable DNA binding and preservation capacity for at least 130 days at room temperature without DNA degradation.

The Structural Characteristics, Management, and Challenges of Backyard Poultry Farming in Residential Areas of Turkey.

The aim of this study was to collect, for the first time, comprehensive information about the backyard poultry sector in Turkey. This included a profile of the poultry owners, flock characteristics, husbandry, housing conditions, the owners' biosecurity measures and the challenges of backyard poultry farming in residential areas of Turkey. An online semi-structured survey was fully completed by 1094 respondents. The majority of respondents (91%) owned fewer than 50 birds and reported raising other poultry species besides chickens (64%). Most of the participants indicated that they kept poultry as food for family use (83%) and had been involved in chicken-raising activities for less than 10 years (86%). Free-range coops were the type of housing most preferred by the respondents (86%). However, there was a lack of awareness about poultry diseases and poultry health care conditions. Respondents that confirmed wild bird and rodent access to their feeders and drinkers reported high rates of internal-external parasites, Escherichia coli infections and chronic respiratory diseases (p < 0.001). Lack of regional regulations (84%), animal theft (80%), lack of information on poultry management (79%), minimizing predation (75%), and the need for vaccination and veterinary services (73%) were the most cited challenges. The results highlighted the need for improved biosecurity measures and for a regulatory framework that takes into account the risks to commercial poultry flocks and public health.