RESUMO
Few molecular studies have identified the current status of cystic echinococcosis in Egypt. The present study aimed to ascertain the genotype(s) of Echinococcus granulosus responsible for human hydatidosis in different Egyptian governorates (regions). Animal isolates were collected from 40 camels, 5 pigs and 44 sheep. 27 human isolates were included in the present study. Specific PCR was performed and followed by DNA sequencing for mitochondrial 12S ribosomal RNA gene and BLAST analysis.The sheep cysts were not hydatid cysts. G6 genotype (camel starin) predominates in human, camel and pig isolates. G7 genotype (pig strain) was detected in two human isolates and one pig isolate. G1 genotype (sheep strain) was detected in one human isolate from Yemen and in no animal isolates. This is the first record of G7 in Egypt and G1 in Yemen.
Assuntos
Equinococose/parasitologia , Equinococose/veterinária , Echinococcus granulosus/classificação , Echinococcus granulosus/genética , Genótipo , Animais , Camelus , Análise por Conglomerados , DNA de Helmintos/genética , Echinococcus granulosus/isolamento & purificação , Egito , Humanos , Reação em Cadeia da Polimerase , RNA Ribossômico/genética , Análise de Sequência de DNA , Homologia de Sequência , Ovinos , Suínos , IêmenRESUMO
Cell-based therapy is emerging as a promising therapeutic approach for a wide range of liver diseases. This study aimed to investigate the regenerative and antifibrotic therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) in an early and late experimental hepatic schistosomiasis model. BM-MSCs were isolated from 6-wk-old BALB/c donor male mice, then grown and propagated in culture until cell count was 5-8 × 10(6)/ml. MSCs were then separated and injected into Schistosoma mansoni -infected female BALB/c mice on their 6, 10, 14, and 18 wk post-infection. Mice were sacrificed on the fourth and eighth week after BM-MSCs transplantation in each group. Homing of BM-MSCs was confirmed by PCR detection of male Y-chromosome gene (sry) in the liver tissue of the recipient female mice. The regenerative and antifibrotic potential of BM-MSCs was assessed by histopathological examination, morphometric analysis, electron microscopy, and liver function tests. Schistosoma-infected mice, which were treated with BM-MSCs, showed a decrease in the granuloma size, percentage and density of the fibrotic area, formation of new hepatocytes, and improvement of the liver function tests. Immunohistochemical examination of alpha-smooth muscle actin revealed a significant decrease in the immunoreactive hepatic stellate cells in mice treated with MSCs. Early granulomas (acute infection) showed better response to MSC injection than did later granulomas (chronic infection). Dosing and timing of MSCs transplantation should undergo more investigations in long-term experiments before application to the clinical field. This study is the first to assess and compare the effect of MSCs treatment on early and late granulomas.
Assuntos
Terapia Baseada em Transplante de Células e Tecidos , Células-Tronco Mesenquimais/fisiologia , Esquistossomose mansoni/terapia , Actinas/análise , Alanina Transaminase/sangue , Animais , Biomphalaria , Terapia Baseada em Transplante de Células e Tecidos/métodos , Terapia Baseada em Transplante de Células e Tecidos/normas , Modelos Animais de Doenças , Feminino , Genes sry/genética , Imuno-Histoquímica , Fígado/química , Fígado/citologia , Fígado/patologia , Fígado/fisiologia , Testes de Função Hepática , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Reação em Cadeia da Polimerase , Albumina Sérica/análiseRESUMO
Ferula assafoetida is a hard, resinous, oily herbaceous gum belonging to plant family Umbelliferae. It is used as a traditional medicine in many parts of the world and its wide use in medicine was listed by many authors. In the current study, the effect of F. assafoetida on Schistosoma mansoni in experimentally infected mice is investigated. F. assafoetida was given orally via intragastric tube in an oil-form and a powder-form in different concentrations. Four test groups of 30 mice each were studied. Gs I & II mice were given F. assafoetida in an oil-form in different concentrations at 4 and 6 weeks post infection (PI) respectively. Mice in Gs III & IV were given re-constituted F. assafoetida powder in different concentrations (conc.) at 4 & 6 weeks PI. respectively like the previous groups. Oil-form F. assafoetida was given at conc. of 50, 25 & 15 mg/ml. Powder-form was given at conc. of 32, 16 & 8 mg/ml. A highly significant statistical difference between the test Gs (I, II, III & IV) was recorded in comparison to the infection control G with p value < 0.0001 and also between powder and oil forms of F. assafoetida (p < 0.0001) as regards the mean worm burden and tissue egg count. The highest reduction in worm burden and egg counts was found with powder form of F. assafoetida (Gs III & IV) when compared to oil form (Gs I & II), as confirmed histopathologically and by ultrastructural profile alteration.