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1.
Stem Cell Reports ; 13(2): 405-418, 2019 08 13.
Artigo em Inglês | MEDLINE | ID: mdl-31353226

RESUMO

Reduction of caloric intake delays and prevents age-associated diseases and extends the life span in many organisms. It may be that these benefits are due to positive effects of caloric restriction on stem cell function. We use the planarian model Schmidtea mediterranea, an immortal animal that adapts to long periods of starvation by shrinking in size, to investigate the effects of starvation on telomere length. We show that the longest telomeres are a general signature of planarian adult stem cells. We also observe that starvation leads to an enrichment of stem cells with the longest telomeres and that this enrichment is dependent on mTOR signaling. We propose that one important effect of starvation for the rejuvenation of the adult stem cell pool is through increasing the median telomere length in somatic stem cells. Such a mechanism has broad implications for how dietary effects on aging are mediated at the whole-organism level.


Assuntos
Planárias/fisiologia , Serina-Treonina Quinases TOR/metabolismo , Telômero/genética , Células-Tronco Adultas/citologia , Células-Tronco Adultas/metabolismo , Animais , Proteínas Argonautas/antagonistas & inibidores , Proteínas Argonautas/genética , Proteínas Argonautas/metabolismo , Regulação para Baixo , Proteínas de Helminto/antagonistas & inibidores , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Modelos Biológicos , Planárias/genética , Interferência de RNA , RNA de Cadeia Dupla/metabolismo , Transdução de Sinais , Inanição , Homeostase do Telômero
2.
Nat Commun ; 9(1): 3633, 2018 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-30194301

RESUMO

Currently, little is known about the evolution of epigenetic regulation in animal stem cells. Here we demonstrate, using the planarian stem cell system to investigate the role of the COMPASS family of MLL3/4 histone methyltransferases that their function as tumor suppressors in mammalian stem cells is conserved over a long evolutionary distance. To investigate the potential conservation of a genome-wide epigenetic regulatory program in animal stem cells, we assess the effects of Mll3/4 loss of function by performing RNA-seq and ChIP-seq on the G2/M planarian stem cell population, part of which contributes to the formation of outgrowths. We find many oncogenes and tumor suppressors among the affected genes that are likely candidates for mediating MLL3/4 tumor suppression function. Our work demonstrates conservation of an important epigenetic regulatory program in animals and highlights the utility of the planarian model system for studying epigenetic regulation.


Assuntos
Epigênese Genética , Evolução Molecular , Histona Metiltransferases/fisiologia , Células-Tronco Pluripotentes/fisiologia , Proteínas Supressoras de Tumor/fisiologia , Animais , Neurogênese , Oncogenes , Planárias , Regeneração
3.
Genome Res ; 28(10): 1543-1554, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30143598

RESUMO

Planarian flatworms have an indefinite capacity to regenerate missing or damaged body parts owing to a population of pluripotent adult stems cells called neoblasts (NBs). Currently, little is known about the importance of the epigenetic status of NBs and how histone modifications regulate homeostasis and cellular differentiation. We have developed an improved and optimized ChIP-seq protocol for NBs in Schmidtea mediterranea and have generated genome-wide profiles for the active marks H3K4me3 and H3K36me3, and suppressive marks H3K4me1 and H3K27me3. The genome-wide profiles of these marks were found to correlate well with NB gene expression profiles. We found that genes with little transcriptional activity in the NB compartment but which switch on in post-mitotic progeny during differentiation are bivalent, being marked by both H3K4me3 and H3K27me3 at promoter regions. In further support of this hypothesis, bivalent genes also have a high level of paused RNA Polymerase II at the promoter-proximal region. Overall, this study confirms that epigenetic control is important for the maintenance of a NB transcriptional program and makes a case for bivalent promoters as a conserved feature of animal stem cells and not a vertebrate-specific innovation. By establishing a robust ChIP-seq protocol and analysis methodology, we further promote planarians as a promising model system to investigate histone modification-mediated regulation of stem cell function and differentiation.


Assuntos
Proteínas de Helminto/genética , Histonas/metabolismo , Planárias/genética , Células-Tronco Pluripotentes/citologia , Animais , Diferenciação Celular , Imunoprecipitação da Cromatina , Epigênese Genética , Perfilação da Expressão Gênica , Código das Histonas , Processamento de Proteína Pós-Traducional , Análise de Sequência de DNA , Análise de Sequência de RNA
4.
Dev Biol ; 433(2): 118-131, 2018 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-29198565

RESUMO

How do animals regenerate specialised tissues or their entire body after a traumatic injury, how has this ability evolved and what are the genetic and cellular components underpinning this remarkable feat? While some progress has been made in understanding mechanisms, relatively little is known about the evolution of regenerative ability. Which elements of regeneration are due to lineage specific evolutionary novelties or have deeply conserved roots within the Metazoa remains an open question. The renaissance in regeneration research, fuelled by the development of modern functional and comparative genomics, now enable us to gain a detailed understanding of both the mechanisms and evolutionary forces underpinning regeneration in diverse animal phyla. Here we review existing and emerging model systems, with the focus on invertebrates, for studying regeneration. We summarize findings across these taxa that tell us something about the evolution of adult stem cell types that fuel regeneration and the growing evidence that many highly regenerative animals harbor adult stem cells with a gene expression profile that overlaps with germline stem cells. We propose a framework in which regenerative ability broadly evolves through changes in the extent to which stem cells generated through embryogenesis are maintained into the adult life history.


Assuntos
Células-Tronco Adultas/fisiologia , Evolução Biológica , Regeneração/fisiologia , Células-Tronco Germinativas Adultas/fisiologia , Animais , Proteínas Argonautas/fisiologia , Linhagem da Célula , Desenvolvimento Embrionário , Humanos , Invertebrados/citologia , Invertebrados/fisiologia , Modelos Animais , Modelos Biológicos , Células-Tronco Multipotentes/fisiologia , Filogenia , Células-Tronco Pluripotentes/fisiologia , RNA Interferente Pequeno/genética , Especificidade da Espécie
5.
Dev Biol ; 433(2): 218-226, 2018 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-28757112

RESUMO

Heterogeneity of planarian stem cells has been categorised on the basis of single cell expression analyses and subsequent experiments to demonstrate lineage relationships. Some data suggest that despite heterogeneity in gene expression amongst cells in the cell cycle, in fact only one sub-population, known as sigma neoblasts, can self-renew. Without the tools to perform live in vivo lineage analysis, we instead took an alternative approach to provide independent evidence for defining the self-renewing stem cell population. We exploited the role of highly conserved condensin family genes to functionally assay neoblast self-renewal properties. Condensins are involved in forming properly condensed chromosomes to allow cell division to proceed during mitosis, and their abrogation inhibits mitosis and can lead to repeated endoreplication of the genome in cells that make repeated attempts to divide. We find that planarians possess only the condensin I complex, and that this is required for normal stem cell function. Abrogation of condensin function led to rapid stem cell depletion accompanied by the appearance of 'giant' cells with increased DNA content. Using previously discovered markers of heterogeneity we show that enlarged cells are always from the sigma-class of the neoblast population and we never observe evidence for endoreplication for the other neoblast subclasses. Overall, our data establish that condensins are essential for stem cell maintenance and provide independent evidence that only sigma-neoblasts are capable of multiple rounds of cell division and hence self-renewal.


Assuntos
Adenosina Trifosfatases/fisiologia , Células-Tronco Adultas/fisiologia , Autorrenovação Celular , Proteínas de Ligação a DNA/fisiologia , Proteínas de Helminto/fisiologia , Complexos Multiproteicos/fisiologia , Planárias/fisiologia , Células-Tronco Pluripotentes/fisiologia , Regeneração/fisiologia , Adenosina Trifosfatases/antagonistas & inibidores , Adenosina Trifosfatases/genética , Animais , Apoptose , Divisão Celular , Segregação de Cromossomos/fisiologia , Proteínas de Ligação a DNA/antagonistas & inibidores , Proteínas de Ligação a DNA/genética , Endorreduplicação , Raios gama , Regulação da Expressão Gênica , Mitose , Complexos Multiproteicos/antagonistas & inibidores , Complexos Multiproteicos/genética , Fenótipo , Filogenia , Planárias/citologia , Planárias/efeitos da radiação , Interferência de RNA , RNA Interferente Pequeno/genética
6.
Development ; 144(19): 3440-3453, 2017 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-28893948

RESUMO

Migration of stem cells underpins the physiology of metazoan animals. For tissues to be maintained, stem cells and their progeny must migrate and differentiate in the correct positions. This need is even more acute after tissue damage by wounding or pathogenic infection. Inappropriate migration also underpins metastasis. Despite this, few mechanistic studies address stem cell migration during repair or homeostasis in adult tissues. Here, we present a shielded X-ray irradiation assay that allows us to follow stem cell migration in planarians. We demonstrate the use of this system to study the molecular control of stem cell migration and show that snail-1, snail-2 and zeb-1 EMT transcription factor homologs are necessary for cell migration to wound sites and for the establishment of migratory cell morphology. We also observed that stem cells undergo homeostatic migration to anterior regions that lack local stem cells, in the absence of injury, maintaining tissue homeostasis. This requires the polarity determinant notum Our work establishes planarians as a suitable model for further in-depth study of the processes controlling stem cell migration in vivo.


Assuntos
Células-Tronco Adultas/citologia , Movimento Celular , Transição Epitelial-Mesenquimal , Planárias/citologia , Planárias/metabolismo , Células-Tronco Pluripotentes/citologia , Fatores de Transcrição/metabolismo , Células-Tronco Adultas/metabolismo , Células-Tronco Adultas/efeitos da radiação , Animais , Linhagem da Célula/efeitos da radiação , Movimento Celular/efeitos da radiação , Forma Celular/efeitos da radiação , Sequência Conservada , Células Epidérmicas , Transição Epitelial-Mesenquimal/efeitos da radiação , Cadeias beta de Integrinas/metabolismo , Metaloproteinases da Matriz/metabolismo , Planárias/genética , Células-Tronco Pluripotentes/efeitos da radiação , Fatores de Transcrição da Família Snail/metabolismo , Raios X
7.
Semin Cell Dev Biol ; 70: 108-121, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28818620

RESUMO

Understanding how some animals are immortal and avoid the ageing process is important. We currently know very little about how they achieve this. Research with genetic model systems has revealed the existence of conserved genetic pathways and molecular processes that affect longevity. Most of these established model organisms have relatively short lifespans. Here we consider the use of planarians, with an immortal life-history that is able to entirely avoid the ageing process. These animals are capable of profound feats of regeneration fueled by a population of adult stem cells called neoblasts. These cells are capable of indefinite self-renewal that has underpinned the evolution of animals that reproduce only by fission, having disposed of the germline, and must therefore be somatically immortal and avoid the ageing process. How they do this is only now starting to be understood. Here we suggest that the evidence so far supports the hypothesis that the lack of ageing is an emergent property of both being highly regenerative and the evolution of highly effective mechanisms for ensuring genome stability in the neoblast stem cell population. The details of these mechanisms could prove to be very informative in understanding how the causes of ageing can be avoided, slowed or even reversed.


Assuntos
Envelhecimento/genética , Reparo do DNA , Genoma , Instabilidade Genômica , Planárias/genética , Regeneração/genética , Homeostase do Telômero , Células-Tronco Adultas/citologia , Células-Tronco Adultas/metabolismo , Processamento Alternativo , Animais , Diferenciação Celular , Proliferação de Células , Replicação do DNA , Modelos Biológicos , Planárias/crescimento & desenvolvimento , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Telomerase/genética , Telomerase/metabolismo , Telômero/química , Telômero/metabolismo
8.
Cell Rep ; 20(1): 173-187, 2017 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-28683311

RESUMO

As core components of the microRNA-induced silencing complex (miRISC), Argonaute (AGO) proteins interact with TNRC6 proteins, recruiting other effectors of translational repression/mRNA destabilization. Here, we show that LIMD1 coordinates the assembly of an AGO-TNRC6 containing miRISC complex by binding both proteins simultaneously at distinct interfaces. Phosphorylation of AGO2 at Ser 387 by Akt3 induces LIMD1 binding, which in turn enables AGO2 to interact with TNRC6A and downstream effector DDX6. Conservation of this serine in AGO1 and 4 indicates this mechanism may be a fundamental requirement for AGO function and miRISC assembly. Upon CRISPR-Cas9-mediated knockout of LIMD1, AGO2 miRNA-silencing function is lost and miRNA silencing becomes dependent on a complex formed by AGO3 and the LIMD1 family member WTIP. The switch to AGO3 utilization occurs due to the presence of a glutamic acid residue (E390) on the interaction interface, which allows AGO3 to bind to LIMD1, AJUBA, and WTIP irrespective of Akt signaling.


Assuntos
Proteínas Argonautas/metabolismo , Inativação Gênica , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteínas com Domínio LIM/metabolismo , MicroRNAs/genética , Proteínas Argonautas/genética , Autoantígenos/metabolismo , RNA Helicases DEAD-box/metabolismo , Células HEK293 , Células HeLa , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/química , Peptídeos e Proteínas de Sinalização Intracelular/genética , Proteínas com Domínio LIM/química , Proteínas com Domínio LIM/genética , MicroRNAs/metabolismo , Fosforilação , Ligação Proteica , Processamento de Proteína Pós-Traducional , Proteínas Proto-Oncogênicas/metabolismo , Proteínas de Ligação a RNA/metabolismo
9.
BMC Genomics ; 18(1): 389, 2017 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-28521727

RESUMO

BACKGROUND: Growing global demands for crustacean food crop species have driven large investments in aquaculture research worldwide. However, large-scale production is susceptible to pathogen-mediated destruction particularly in developing economies. Thus, a thorough understanding of the immune system components of food crop species is imperative for research to combat pathogens. RESULTS: Through a comparative genomics approach utilising extant data from 55 species, we describe the innate immune system of the class Malacostraca, which includes all food crop species. We identify 7407 malacostracan genes from 39 gene families implicated in different aspects of host defence and demonstrate dynamic evolution of innate immunity components within this group. Malacostracans have achieved flexibility in recognising infectious agents through divergent evolution and expansion of pathogen recognition receptors genes. Antiviral RNAi, Toll and JAK-STAT signal transduction pathways have remained conserved within Malacostraca, although the Imd pathway appears to lack several key components. Immune effectors such as the antimicrobial peptides (AMPs) have unique evolutionary profiles, with many malacostracan AMPs not found in other arthropods. Lastly, we describe four putative novel immune gene families, potentially representing important evolutionary novelties of the malacostracan immune system. CONCLUSION: Our analyses across the broader Malacostraca have allowed us to not only draw analogies with other arthropods but also to identify evolutionary novelties in immune modulation components and form strong hypotheses as to when key pathways have evolved or diverged. This will serve as a key resource for future immunology research in crustacean food crops.


Assuntos
Sequência Conservada , Crustáceos/genética , Crustáceos/imunologia , Evolução Molecular , Genômica , Imunidade Inata/genética , Animais , Crustáceos/citologia , Crustáceos/virologia , Transdução de Sinais/genética
10.
BMC Evol Biol ; 17(1): 107, 2017 04 26.
Artigo em Inglês | MEDLINE | ID: mdl-28441946

RESUMO

BACKGROUND: Most animals employ telomerase, which consists of a catalytic subunit known as the telomerase reverse transcriptase (TERT) and an RNA template, to maintain telomere ends. Given the importance of TERT and telomere biology in core metazoan life history traits, like ageing and the control of somatic cell proliferation, we hypothesised that TERT would have patterns of sequence and regulatory evolution reflecting the diverse life histories across the Animal Kingdom. RESULTS: We performed a complete investigation of the evolutionary history of TERT across animals. We show that although TERT is almost ubiquitous across Metazoa, it has undergone substantial sequence evolution within canonical motifs. Beyond the known canonical motifs, we also identify and compare regions that are highly variable between lineages, but show conservation within phyla. Recent data have highlighted the importance of alternative splice forms of TERT in non-canonical functions and although animals may share some conserved introns, we find that the selection of exons for alternative splicing appears to be highly variable, and regulation by alternative splicing appears to be a very dynamic feature of TERT evolution. We show that even within a closely related group of triclad flatworms, where alternative splicing of TERT was previously correlated with reproductive strategy, we observe highly diverse splicing patterns. CONCLUSIONS: Our work establishes that the evolutionary history and structural evolution of TERT involves previously unappreciated levels of change and the emergence of lineage specific motifs. The sequence conservation we describe within phyla suggests that these new motifs likely serve essential biological functions of TERT, which along with changes in splicing, underpin diverse functions of TERT important for animal life histories.


Assuntos
Evolução Molecular , Telomerase/química , Telomerase/genética , Processamento Alternativo , Animais , Domínio Catalítico , Sequência Conservada , Éxons , Íntrons , Filogenia , Subunidades Proteicas/genética , Telômero/genética , Telômero/metabolismo
11.
Development ; 142(14): 2413-24, 2015 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-26062938

RESUMO

Regeneration involves the integration of new and old tissues in the context of an adult life history. It is clear that the core conserved signalling pathways that orchestrate development also play central roles in regeneration, and further study of conserved signalling pathways is required. Here we have studied the role of the conserved JNK signalling cascade during planarian regeneration. Abrogation of JNK signalling by RNAi or pharmacological inhibition blocks posterior regeneration and animals fail to express posterior markers. While the early injury-induced expression of polarity markers is unaffected, the later stem cell-dependent phase of posterior Wnt expression is not established. This defect can be rescued by overactivation of the Hh or Wnt signalling pathway to promote posterior Wnt activity. Together, our data suggest that JNK signalling is required to establish stem cell-dependent Wnt expression after posterior injury. Given that Jun is known to be required in vertebrates for the expression of Wnt and Wnt target genes, we propose that this interaction may be conserved and is an instructive part of planarian posterior regeneration.


Assuntos
Regulação da Expressão Gênica , MAP Quinase Quinase 4/metabolismo , Planárias/metabolismo , Transdução de Sinais , Células-Tronco/citologia , Proteínas Wnt/metabolismo , Animais , Padronização Corporal/genética , Diferenciação Celular/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Genoma , Sistema de Sinalização das MAP Quinases/genética , Fenótipo , Planárias/fisiologia , Interferência de RNA , Regeneração
12.
PeerJ ; 2: e382, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24860704

RESUMO

The immune system of the horse has not been well studied, despite the fact that the horse displays several features such as sensitivity to bacterial lipopolysaccharide that make them in many ways a more suitable model of some human disorders than the current rodent models. The difficulty of working with large animal models has however limited characterisation of gene expression in the horse immune system with current annotations for the equine genome restricted to predictions from other mammals and the few described horse proteins. This paper outlines sequencing of 184 million transcriptome short reads from immunologically active tissues of three horses including the genome reference "Twilight". In a comparison with the Ensembl horse genome annotation, we found 8,763 potentially novel isoforms.

13.
PLoS Genet ; 9(12): e1004003, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24367277

RESUMO

Post-transcriptional regulatory mechanisms are of fundamental importance to form robust genetic networks, but their roles in stem cell pluripotency remain poorly understood. Here, we use freshwater planarians as a model system to investigate this and uncover a role for CCR4-NOT mediated deadenylation of mRNAs in stem cell differentiation. Planarian adult stem cells, the so-called neoblasts, drive the almost unlimited regenerative capabilities of planarians and allow their ongoing homeostatic tissue turnover. While many genes have been demonstrated to be required for these processes, currently almost no mechanistic insight is available into their regulation. We show that knockdown of planarian Not1, the CCR4-NOT deadenylating complex scaffolding subunit, abrogates regeneration and normal homeostasis. This abrogation is primarily due to severe impairment of their differentiation potential. We describe a stem cell specific increase in the mRNA levels of key neoblast genes after Smed-not1 knock down, consistent with a role of the CCR4-NOT complex in degradation of neoblast mRNAs upon the onset of differentiation. We also observe a stem cell specific increase in the frequency of longer poly(A) tails in these same mRNAs, showing that stem cells after Smed-not1 knock down fail to differentiate as they accumulate populations of transcripts with longer poly(A) tails. As other transcripts are unaffected our data hint at a targeted regulation of these key stem cell mRNAs by post-transcriptional regulators such as RNA-binding proteins or microRNAs. Together, our results show that the CCR4-NOT complex is crucial for stem cell differentiation and controls stem cell-specific degradation of mRNAs, thus providing clear mechanistic insight into this aspect of neoblast biology.


Assuntos
Proteínas de Bactérias/genética , Diferenciação Celular/genética , Planárias/genética , Estabilidade de RNA/genética , Regeneração/genética , Ribonucleases/genética , Animais , Proliferação de Células , Regulação da Expressão Gênica no Desenvolvimento , Planárias/crescimento & desenvolvimento , Interferência de RNA , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Células-Tronco/citologia , Células-Tronco/metabolismo
14.
PLoS One ; 8(6): e65170, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23755189

RESUMO

The importance of microRNAs in development is now widely accepted. However, identifying the specific targets of individual microRNAs and understanding their biological significance remains a major challenge. We have used the zebrafish model system to evaluate the expression and function of microRNAs potentially involved in muscle development and study their interaction with predicted target genes. We altered expression of the miR-30 microRNA family and generated phenotypes that mimicked misregulation of the Hedgehog pathway. Inhibition of the miR-30 family increases activity of the pathway, resulting in elevated ptc1 expression and increased numbers of superficial slow-muscle fibres. We show that the transmembrane receptor smoothened is a target of this microRNA family. Our results indicate that fine coordination of smoothened activity by the miR-30 family allows the correct specification and differentiation of distinct muscle cell types during zebrafish embryonic development.


Assuntos
Proteínas Hedgehog/metabolismo , MicroRNAs/genética , Desenvolvimento Muscular/genética , Receptores Acoplados a Proteínas G/genética , Proteínas de Peixe-Zebra/genética , Peixe-Zebra/embriologia , Regiões 3' não Traduzidas , Animais , Sequência de Bases , Sítios de Ligação , Padronização Corporal/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Membrana , Músculo Esquelético/citologia , Músculo Esquelético/embriologia , Músculo Esquelético/metabolismo , Receptores Patched , Receptor Patched-1 , Interferência de RNA , Receptores de Superfície Celular/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Transdução de Sinais , Receptor Smoothened , Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismo
15.
Genome Biol ; 14(2): 106, 2013 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-23425551

RESUMO

The newt transcriptome opens up many new possibilities in the study of regeneration, and the novel gene families identified shed light on lineage-specific mechanisms.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Proteoma/metabolismo , Regeneração/genética , Transcriptoma , Animais
16.
Development ; 140(4): 730-9, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23318635

RESUMO

Recent advances in a number of systems suggest many genes involved in orchestrating regeneration are redeployed from similar processes in development, with others being novel to the regeneration process in particular lineages. Of particular importance will be understanding the architecture of regenerative genetic regulatory networks and whether they are conserved across broad phylogenetic distances. Here, we describe the role of the conserved TALE class protein PBX/Extradenticle in planarians, a representative member of the Lophotrocozoa. PBX/Extradenticle proteins play central roles in both embryonic and post-embryonic developmental patterning in both vertebrates and insects, and we demonstrate a broad requirement during planarian regeneration. We observe that Smed-pbx has pleiotropic functions during regeneration, with a primary role in patterning the anterior-posterior (AP) axis and AP polarity. Smed-pbx is required for expression of polarity determinants notum and wnt1 and for correct patterning of the structures polarized along the AP axis, such as the brain, pharynx and gut. Overall, our data suggest that Smed-pbx functions as a central integrator of positional information to drive patterning of regeneration along the body axis.


Assuntos
Padronização Corporal/fisiologia , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Redes Reguladoras de Genes/fisiologia , Proteínas de Homeodomínio/fisiologia , Planárias/fisiologia , Regeneração/fisiologia , Fatores de Transcrição/fisiologia , Animais , Sequência de Bases , Clonagem Molecular , Primers do DNA/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Homeodomínio/genética , Imuno-Histoquímica , Hibridização In Situ , Microscopia de Fluorescência , Dados de Sequência Molecular , Faringe/cirurgia , Interferência de RNA , Análise de Sequência de DNA , Fatores de Transcrição/genética
17.
Virology ; 433(1): 55-63, 2012 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-22868041

RESUMO

The recently released draft horse genome is incompletely characterised in terms of its repetitive element profile. This paper presents characterisation of the endogenous retrovirus (ERVs) of the horse genome based on a data-mining strategy using murine leukaemia virus proteins as queries. 978 ERV gene sequences were identified. Sequences were identified from the gamma, epsilon and betaretrovirus genera. At least one full length gammaretroviral locus was identified, though the gammaretroviral sequences are very degenerate. Using these data the RNA expression of these ERVs were derived from RNA transcriptome data from a variety of equine tissues. Unlike the well studied human and murine ERVs there do not appear to be particular phylogenetic groups of equine ERVs that are more transcriptionally active. Using this novel approach provided a more technically feasible method to characterise ERV expression than previous studies.


Assuntos
Retrovirus Endógenos/genética , Genoma , Cavalos/genética , Cavalos/virologia , Animais , Betaretrovirus/genética , Mineração de Dados , Retrovirus Endógenos/classificação , Epsilonretrovirus/genética , Gammaretrovirus/genética , Camundongos , Filogenia , Transcrição Gênica , Transcriptoma
18.
Methods Mol Biol ; 916: 167-79, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22914940

RESUMO

Planarians provide a relatively simple model system in which to study stem cell dynamics and regenerative phenomena. As with other systems understanding the dynamics of stem cell and stem cell progeny is crucial in order to get at the molecular mechanisms orchestrating stem cell biology. Planarians have an abundant adult stem cell population that can be observed using Fluorescence-Activated Cell Sorting (FACS). This approach allows different subpopulations of stem cells and their progeny to be monitored and sorted for downstream studies in response to different regenerative scenarios, drug treatments, or RNAi knockdown of genes required for regenerative events.


Assuntos
Citometria de Fluxo/métodos , Planárias/citologia , Planárias/fisiologia , Regeneração , Células-Tronco/citologia , Animais , Técnicas de Cultura , Planárias/crescimento & desenvolvimento , Coloração e Rotulagem , Células-Tronco/metabolismo
19.
EMBO J ; 31(12): 2747-9, 2012 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-22562151

RESUMO

Stem cells, both adult and germline, are the key cells underpinning animal evolution. Yet, surprisingly little is known about the evolution of their shared key feature: pluripotency. Now using genome-wide expression profiling of pluripotent planarian adult stem cells (pASCs), Önal et al (2012) present evidence for deep molecular conservation of pluripotency. They characterise the expression profile of pASCs and identify conserved expression profiles and functions for genes required for mammalian pluripotency. Their analyses suggest that molecular pluripotency mechanisms may be conserved, and tantalisingly that pluripotency in germ stem cells (GSCs) and somatic stem cells (SSCs) may have had shared common evolutionary origins.


Assuntos
Diferenciação Celular , Proliferação de Células , Regulação da Expressão Gênica , Planárias/citologia , Células-Tronco Pluripotentes/fisiologia , Animais
20.
PLoS Genet ; 8(3): e1002619, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22479207

RESUMO

Planarian flatworms are able to both regenerate their whole bodies and continuously adapt their size to nutrient status. Tight control of stem cell proliferation and differentiation during these processes is the key feature of planarian biology. Here we show that the planarian homolog of the phosphoinositide 3-kinase-related kinase (PIKK) family member SMG-1 and mTOR complex 1 components are required for this tight control. Loss of smg-1 results in a hyper-responsiveness to injury and growth and the formation of regenerative blastemas that remain undifferentiated and that lead to lethal ectopic outgrowths. Invasive stem cell hyper-proliferation, hyperplasia, hypertrophy, and differentiation defects are hallmarks of this uncontrolled growth. These data imply a previously unappreciated and novel physiological function for this PIKK family member. In contrast we found that planarian members of the mTOR complex 1, tor and raptor, are required for the initial response to injury and blastema formation. Double smg-1 RNAi experiments with tor or raptor show that abnormal growth requires mTOR signalling. We also found that the macrolide rapamycin, a natural compound inhibitor of mTORC1, is able to increase the survival rate of smg-1 RNAi animals by decreasing cell proliferation. Our findings support a model where Smg-1 acts as a novel regulator of both the response to injury and growth control mechanisms. Our data suggest the possibility that this may be by suppressing mTOR signalling. Characterisation of both the planarian mTORC1 signalling components and another PIKK family member as key regulators of regeneration and growth will influence future work on regeneration, growth control, and the development of anti-cancer therapies that target mTOR signalling.


Assuntos
Planárias , Regeneração , Células-Tronco , Serina-Treonina Quinases TOR/genética , Animais , Diferenciação Celular , Proliferação de Células/efeitos dos fármacos , Humanos , Dados de Sequência Molecular , Fosfatidilinositol 3-Quinases/genética , Planárias/genética , Planárias/crescimento & desenvolvimento , Interferência de RNA/efeitos dos fármacos , Regeneração/genética , Transdução de Sinais/efeitos dos fármacos , Sirolimo/farmacologia , Células-Tronco/metabolismo , Serina-Treonina Quinases TOR/antagonistas & inibidores , Serina-Treonina Quinases TOR/metabolismo
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